A Shorter Equilibration Period Improves Post-Warming Outcomes after Vitrification and in Straw Dilution of In Vitro-Produced Bovine Embryos
Martínez-Rodero, Iris (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Ordóñez-León, Erika Alina (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Vendrell-Flotats, Meritxell (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Hidalgo Ordóñez, Carlos Olegario (Servei Regional de Recerca i Desenvolupament Agroalimentari d'Astúries. Departament de selecció i reproducció d'animals)
Esmoris, Joseba (Aliança Basca de Recerca i Tecnologia (BRTA))
Mendibil, Xabier (Aliança Basca de Recerca i Tecnologia (BRTA))
Azcarate, Sabino (Aliança Basca de Recerca i Tecnologia (BRTA))
López Béjar, Manel (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Yeste Oliveras, Marc (Universitat de Girona. Departament de Biologia)
Mogas Amorós, Teresa (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Garcia Martinez, Tania (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Fecha:	2021
Resumen:	This study was designed to the optimize vitrification and in-straw warming protocol of in vitro-produced bovine embryos by comparing two different equilibration periods, short equilibrium (SE: 3 min) and long equilibrium (LE: 12 min). Outcomes recorded in vitrified day seven (D7) and day eight (D8) expanded blastocysts were survival and hatching rates, cell counts, apoptosis rate, and gene expression. While survival rates at 3 and 24 h post-warming were reduced (p < 0. 05) after vitrification, the hatching rates of D7 embryos vitrified after SE were similar to the rates recorded in fresh non-vitrified blastocysts. The hatching rates of vitrified D8 blastocysts were lower (p < 0. 05) than of fresh controls regardless of treatment. Total cell count, and inner cell mass and trophectoderm cell counts were similar in hatched D7 blastocysts vitrified after SE and fresh blastocysts, while vitrified D8 blastocysts yielded lower values regardless of treatment. The apoptosis rate was significantly higher in both treatment groups compared to fresh controls, although rates were lower for SE than LE. No differences emerged in BAX, AQP3, CX43, and IFNτ gene expression between the treatments, whereas a significantly greater abundance of BCL2L1 and SOD1 transcripts was observed in blastocysts vitrified after SE. A shorter equilibration vitrification protocol was found to improve post-warming outcomes and time efficiency after in-straw warming/dilution.
Ayudas:	Ministerio de Ciencia e Innovación AGL2016-79802-P Agència de Gestió d'Ajuts Universitaris i de Recerca 2017-SGR-1229 Ministerio de Ciencia e Innovación BES-2017-081962
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Cow ; Cryopreservation ; Expanded blastocyst ; Total cell number ; Inner cell mass ; Trophectoderm; ; SOX2 ; TUNEL ; Apoptosis ; Gene expression
Publicado en:	Biology, Vol. 10 Núm. 2 (2021) , p. 142, ISSN 2079-7737

DOI: 10.3390/biology10020142
PMID: 33579034

16 p, 1.4 MB

El registro aparece en las colecciones:
Artículos > Artículos de investigación
Artículos > Artículos publicados