Transmission of Similar Mcr-1 Carrying Plasmids among Different Escherichia coli Lineages Isolated from Livestock and the Farmer
Viñes, Joaquim (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM))
Saez-Llorente, Jose Luis (Ministerio de Agricultura, Pesca y Alimentación. Area de Programas Sanitarios y Zoonosis)
Rosàs-Rodoreda, Montserrat (Servei d'Alimentació Animal i Seguretat de la Producció Ramadera. Departament d'Agricultura, Ramaderia, Pesca i Alimentació)
Francino, Olga (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM))
Migura-Garcia, Lourdes (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Cuscó, Anna (Universitat Autònoma de Barcelona. Vetgenomics)
Napp Avelli, Sebastián (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Álvarez, Julio (Universidad Complutense de Madrid. Departamento de Sanidad Animal)

Date:	2021
Abstract:	Colistin use has mostly been stopped in human medicine, due to its toxicity. However, nowadays, it still is used as a last-resort antibiotic to treat hospital infections caused by multi-drug resistant Enterobacteriaceae. On the contrary, colistin has been used in veterinary medicine until recently. In this study, 210 fecal samples from pigs (n = 57), calves (n = 152), and the farmer (n = 1) were collected from a farm where E. coli harboring mcr-1-mcr-3 was previously detected. Samples were plated, and mcr-genes presence was confirmed by multiplex-PCR. Hybrid sequencing which determined the presence and location of mcr-1, other antibiotic resistance genes, and virulence factors. Eighteen colistin resistant isolates (13 from calves, four from pigs, and one from the farmer) contained mcr-1 associated with plasmids (IncX4, IncI2, and IncHI2), except for two that yielded mcr-1 in the chromosome. Similar plasmids were distributed in different E. coli lineages. Transmission of mcr-1 to the farmer most likely occurred by horizontal gene transfer from E. coli of calf origin, since plasmids were highly similar (99% coverage, 99. 97% identity). Moreover, 33 virulence factors, including stx2 for Shiga toxin E. coli (STEC) were detected, highlighting the role of livestock as a reservoir of pathotypes with zoonotic potential.
Grants:	Ministerio de Economía y Competitividad RTI2018-095586-B-C22
Note:	Altres ajuts: GC/2017DI037
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Escherichia coli ; Colistin ; Mcr ; Plasmids ; MinION nanopore ; Hybrid sequencing ; Livestock
Published in:	Antibiotics, Vol. 10 Núm. 3 (2021) , p. 313, ISSN 2079-6382

DOI: 10.3390/antibiotics10030313
PMID: 33803068

13 p, 3.9 MB

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