Fungal bioremediation of diuron-contaminated waters : evaluation of its degradation and the effect of amendable factors on its removal in a trickle-bed reactor under non-sterile conditions
Hu, Kaidi (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Torán, Josefina (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
López-García, Ester (Institut de Diagnosi Ambiental i Estudis de l'Aigua)
Barbieri, Maria Vittoria (Institut de Diagnosi Ambiental i Estudis de l'Aigua)
Postigo, Cristina (Institut de Diagnosi Ambiental i Estudis de l'Aigua)
López de Alda, Miren (Institut de Diagnosi Ambiental i Estudis de l'Aigua)
Caminal i Saperas, Glòria (Institut de Química Avançada de Catalunya)
Sarrà i Adroguer, Montserrat (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Blánquez Cano, Paqui (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)

Fecha:	2020
Resumen:	The occurrence of the extensively used herbicide diuron in the environment poses a severe threat to the ecosystem and human health. Four different ligninolytic fungi were studied as biodegradation candidates for the removal of diuron. Among them, T. versicolor was the most effective species, degrading rapidly not only diuron (83%) but also the major metabolite 3,4-dichloroaniline (100%), after 7-day incubation. During diuron degradation, five transformation products (TPs) were found to be formed and the structures for three of them are tentatively proposed. According to the identified TPs, a hydroxylated intermediate 3-(3,4-dichlorophenyl)-1-hydroxymethyl-1-methylurea (DCPHMU) was further metabolized into the N-dealkylated compounds 3-(3,4-dichlorophenyl)-1-methylurea (DCPMU) and 3,4-dichlorophenylurea (DCPU). The discovery of DCPHMU suggests a relevant role of hydroxylation for subsequent N-demethylation, helping to better understand the main reaction mechanisms of diuron detoxification. Experiments also evidenced that degradation reactions may occur intracellularly and be catalyzed by the cytochrome P450 system. A response surface method, established by central composite design, assisted in evaluating the effect of operational variables in a trickle-bed bioreactor immobilized with T. versicolor on diuron removal. The best performance was obtained at low recycling ratios and influent flow rates. Furthermore, results indicate that the contact time between the contaminant and immobilized fungi plays a crucial role in diuron removal. This study represents a pioneering step forward amid techniques for bioremediation of pesticides-contaminated waters using fungal reactors at a real scale.
Ayudas:	Ministerio de Economía y Competitividad CTM2016-75587-C2-1-R Ministerio de Economía y Competitividad CTM2016-75587-C2-2-R European Commission 727450 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-14 Ministerio de Ciencia e Innovación CEX2018-000794-S
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió acceptada per publicar
Materia:	White-rot fungi ; Herbicide removal ; Transformation products ; Bioreactor ; Response surface methodology ; High-resolution mass spectrometry
Publicado en:	Science of the total environment, Vol. 743 (November 2020) , art. 140628, ISSN 1879-1026

DOI: 10.1016/j.scitotenv.2020.140628
PMID: 32652358

Postprint 40 p, 958.6 KB

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