Reply to Detection of Leptospira interrogans DNA in Urine of a Captive Ocelot (Leopardus pardalis)
Murillo Picco, Andrea (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Cuenca Valera, Rafaela (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Serrano Ferron, Emmanuel (Universitat Autònoma de Barcelona. Grup de Recerca Wildlife Ecology & Health)
Marga, Goris (Amsterdam Universitair Medische Centra (Amsterdam, Països Baixos))
Ahmed, Ahmed (Amsterdam Universitair Medische Centra (Amsterdam, Països Baixos))
Cervantes, Salvador (Clínica Felina Barcelona)
Caparrós, Cristina (Clínica Felina Barcelona)
Vieitez, Verónica (Universidad de Extremadura. Facultad de Veterinaria)
Ladina, Andrea (Universidad de Extremadura. Facultad de Veterinaria)
Pastor Milán, Josep (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Fecha:	2021
Resumen:	In reply to a comment, we want to mention that we consider this report significant in animal leptospirosis. The presence of leptospiral DNA in the urine of a captive wild cat supports findings described in previous reports [1,2], as in our study, where leptospiral DNA is detected in urine without the presence of anti-leptospiral antibodies. This finding further increases the likelihood that domestic and wild cats play a key role in the maintenance and transmission of Leptospira spp. The methodology used in this work was similar to ours. Concerning the MAT technique, we would like to mention that the panel of serovars used was wide and followed the suggestions of the World Organization for Animal Health. There are differences in some of the serovars used between studies, but this is normal. For the accurate diagnostic and epidemiological surveillance of leptospirosis, it is imperative to use endemic serovars in the geographic region. Concerning the PCR methodology, it was not only used for the detection of the lipL32 gene (present only in pathogenic Leptospira spp. ), one step further was taken, and a sequence that allowed the identification of the pathogenic Leptospira spp. as Leptospira interrogans was made. In conclusion, we are aware that this type of research helps us to improve our knowledge about leptospirosis in terms of its epidemiology and reservoir animals.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Publicado en:	International journal of environmental research and public health, Vol. 18 (january 2021) , ISSN 1660-4601

DOI: 10.3390/ijerph18020796
PMID: 33477767

1 p, 266.9 KB

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