Effects of titanium dioxide nanoparticles on the Hprt gene mutations in V79 hamster cells
Kazimirova, Alena (Slovak Medical University. Department of Biology)
El Yamani, Naouale (Norwegian Institute for Air Research. Department of Environmental Chemistry)
Rubio Lorente, Laura (Universidad Católica Madre y Maestra. Laboratorio de Nanobiología)
García-Rodríguez, Alba (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Barančoková, Magdalena (Slovak Medical University. Department of Biology)
Marcos Dauder, Ricardo (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Dusinska, Maria (Norwegian Institute for Air Research. Department of Environmental Chemistry)

Date:	2020
Abstract:	The genotoxicity of anatase/rutile TiO nanoparticles (TiO NPs, NM105 at 3, 15 and 75 µg/cm 2) was assessed with the mammalian in-vitro Hypoxanthine guanine phosphoribosyl transferase (Hprt) gene mutation test in Chinese hamster lung (V79) fibroblasts after 24 h exposure. Two dispersion procedures giving different size distribution and dispersion stability were used to investigate whether the effects of TiO NPs depend on the state of agglomeration. TiO NPs were fully characterised in the previous European FP7 projects NanoTEST and NanoREG2. Uptake of TiO NPs was measured by transmission electron microscopy (TEM). TiO NPs were found in cytoplasmic vesicles, as well as close to the nucleus. The internalisation of TiO NPs did not depend on the state of agglomeration and dispersion used. The cytotoxicity of TiO NPs was measured by determining both the relative growth activity (RGA) and the plating efficiency (PE). There were no substantial effects of exposure time (24, 48 and 72 h), although a tendency to lower RGA at longer exposure was observed. No significant difference in PE values and no increases in the Hprt gene mutant frequency were found in exposed relative to unexposed cultures in spite of evidence of uptake of NPs by cells.
Grants:	European Commission 814425 European Commission 310584 European Commission 262163
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Titanium dioxide nanoparticles ; V79 cells ; Genotoxicity ; Hprt
Published in:	Nanomaterials, Vol. 10, issue 3 (March 2020) , art. 465, ISSN 2079-4991

DOI: 10.3390/nano10030465
PMID: 32150818

12 p, 1.0 MB

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