1 H NMR serum metabolomic profiling of patients at risk of cardiovascular diseases performing stress test
Lema, Camila (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Andrés Villareal, Mireia (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Aguadé-Bruix, Santiago (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Consegal, Marta (Centro de Investigación Biomédica en Red en Enfermedades Cardiovasculares)
Rodriguez-Sinovas, Antonio (Centro de Investigación Biomédica en Red en Enfermedades Cardiovasculares)
Benito, Begoña (Centro de Investigación Biomédica en Red en Enfermedades Cardiovasculares)
Ferreira-Gonzalez, Ignacio (Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública)
Barba, Ignasi (Vall d'Hebron Institut d'Oncologia)
Universitat Autònoma de Barcelona

Date:	2020
Abstract:	Cardiovascular diseases are the leading cause of death worldwide. Changes in lifestyle and/or pharmacological treatment are able to reduce the burden of coronary artery diseases (CAD) and early diagnosis is crucial for the timely and optimal management of the disease. Stress testing is a good method to measure the burden of CAD but it is time consuming and pharmacological testing may not fully mimic exercise test. The objectives of the present project were to characterize the metabolic profile of the population undergoing pharmacological and exercise stress testing to evaluate possible differences between them, and to assess the capacity of 1 H NMR spectroscopy to predict positive stress testing. Pattern recognition was applied to 1 H NMR spectra from serum of patients undergoing stress test and metabolites were quantified. The effects of the stress test, confounding variables and the ability to predict ischemia were evaluated using OPLS-DA. There was an increase in lactate and alanine concentrations in post-test samples in patients undergoing exercise test, but not in those submitted to pharmacological testing. However, when considering only pharmacological patients, those with a positive test result, showed increased serum lactate, that was masked by the much larger amount of lactate associated to exercise testing. In conclusion, we have established that pharmacological stress test does not reproduce the dynamic changes observed in exercise stress. Although there is promising evidence suggesting that 1 H NMR based metabolomics could predict stress test results, further studies with much larger populations will be required in order to obtain a definitive answer.
Grants:	Ministerio de Economía y Competitividad PI14/01431 Instituto de Salud Carlos III PI17/01397 Agència de Gestió d'Ajuts Universitaris i de Recerca SGR-1807
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Acute coronary syndromes ; Metabolomics
Published in:	Scientific reports, Vol. 10 (october 2020) , ISSN 2045-2322

DOI: 10.1038/s41598-020-74880-6
PMID: 33082494

10 p, 1.3 MB

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