Transcriptomic analysis of micropapillary high grade T1 urothelial bladder cancer
Bowden, Michaela (Dana-Farber Cancer Institute (Boston, Estats Units d'Amèrica))
Nadal Rios, Rosa (National Institutes of Health, Bethesda, USA)
Zhou, Chensheng W. (Dana-Farber Cancer Institute (Boston, Estats Units d'Amèrica))
Werner, Lillian (Dana-Farber Cancer Institute (Boston, Estats Units d'Amèrica))
Barletta, Justine (Brigham and Women's Hospital (Boston, Estats Units d'Amèrica))
Juanpere, Nuria (Universitat Pompeu Fabra)
Lloreta, Josep 1958- (Universitat Pompeu Fabra)
Hernandez-Llodrà, Silvia (Universitat Pompeu Fabra)
Morote Robles, Juan (Universitat Autònoma de Barcelona. Departament de Cirurgia)
de Torres, Inés (Hospital Universitari Vall d'Hebron)
Orsola, Anna (Institut Hospital del Mar d'Investigacions Mèdiques)
Cejas, Paloma (Dana Farber Cancer Institute, Boston, USA)
Long, Henry (Dana Farber Cancer Institute, Boston, USA)
Bellmunt, Joaquim (Harvard Medical School, Boston, USA)

Fecha:	2020
Resumen:	No consensus currently exist on the optimal treatment of patients with high-risk nonmuscle invasive (HGT1) micropapillary variant of bladder cancer (MPBC). Transcripsome analysis may allow stratification of MPBC-HGT1 enabling prediction of recurrence and guide therapeutic management for individual patients. Whole transcriptome RNA-Sequencing of tumors from 23 patients with MPBC-HGT1 and 64 conventional urothelial carcinomas (cUC) (reference set) was performed. Differentially expressed genes between MPBC-HGT1 and cUC-HGT1 were explored. Cox proportional hazard models and Kapplan-Meier methods were used to assess the relation between time to progression (TTP) and individual gene expression adjusting for clinical covariates. Over 3000 genes were differentially expressed in MPBC-HGT1 as compared with cUC-HGT1 and a 26-gene signature is characteristic of MPBC within HGT1. A set of three genes; CD36, FAPB3 and RAETE1 ; were significantly associated with TTP. High expression of FABP3 and CD36 were associated with shorter TTP (p = 0. 045 and p = 0. 08) as was low expression of RAET1E (p = 0. 01). Our study suggest that a 26-gene signature can define MPBC-HGT1 within conventional urothelial carcinomas. A prognostic risk index of three genes (FABP3, CD36 and RAET1E) was found to be associated with shorter TTP and may help classify a group of patients with MPBC-HGT1 with high-risk of early progression. These observations might have implications in terms of radical cystectomy recommendation in MPBC patients.
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Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Biomarkers ; Medical research ; Molecular medicine ; Oncology
Publicado en:	Scientific reports, Vol. 10 (november 2020) , ISSN 2045-2322

DOI: 10.1038/s41598-020-76904-7
PMID: 33208770

10 p, 1.8 MB

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