Bicarbonate-Triggered In Vitro Capacitation of Boar Spermatozoa Conveys an Increased Relative Abundance of the Canonical Transient Receptor Potential Cation (TRPC) Channels 3, 4, 6 and 7 and of CatSper-γ Subunit mRNA Transcripts
Lacalle, Estíbaliz (Linköping University. Department of Biomedical & Clinical Sciences)
Consuegra, César (Universidad de Córdoba. Departamento de Medicina y Cirugía Animal)
Martinez, Cristina A.. (Linköping University. Department of Biomedical & Clinical Sciences)
Hidalgo Prieto, Manuel (Universidad de Córdoba. Departamento de Medicina y Cirugía Animal)
Dorado, Jesús (Universidad de Córdoba. Departamento de Medicina y Cirugía Animal)
Martínez-Pastor, Felipe (Universidad de León. Departamento de Biología Molecular)
Álvarez-Rodríguez, Manuel (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Rodriguez-Martinez, Heriberto (Linköping University. Department of Biomedical & Clinical Sciences)

Data:	2022
Resum:	Sperm capacitation is a stepwise complex biochemical process towards fertilization. It includes a crucial early calcium (Ca2+) transport mediated by CatSper channels and Canonical Transient Potential Channels (TRPC). We studied the relative abundance of mRNA transcripts changes of the CatSper β, γ and δ subunits and TRPC-channels 1, 3, 4, 6 and 7 in pig spermatozoa, after triggering in vitro capacitation by bicarbonate ions at levels present in vivo at the fertilization site. For this purpose, we analyzedfive5 ejaculate pools (from three fertile adult boars) before (control-fresh samples) and after in vitro exposure to capacitation conditions (37 mM NaHCO3, 2. 25 mM CaCl2, 2 mM caffeine, 0. 5% bovine serum albumin and 310 mM lactose) at 38 °C, 5% CO2 for 30 min. In vitro capacitation using bicarbonate elicits an increase in the relative abundance of mRNA transcripts of almost all studied Ca2+ channels, except CatSper-δ and TRPC1 (significantly reduced). These findings open new avenues of research to identify the specific role of each channel in boar sperm capacitation and elucidate the physiological meaning of the changes on sperm mRNA cargo.
Ajuts:	Ministerio de Ciencia e Innovación IJCI-2015-24380 Ministerio de Ciencia e Innovación PID2019-108320RJ-I00 Ministerio de Educación, Cultura y Deporte 16/05745 Ministerio de Ciencia e Innovación RTI2018-095183-B-I00
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; Versió publicada
Matèria:	Calcium channels ; CatSper subunits ; TRPC ; Pig ; Capacitation
Publicat a:	Animals, Vol. 12 Núm. 8 (2022) , p. 1012, ISSN 2076-2615

DOI: 10.3390/ani12081012
PMID: 35454259

14 p, 1.1 MB

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