Combination of Two Synchrotron Radiation-Based Techniques and Chemometrics to Study an Enhanced Natural Remineralization of Enamel
Diez-García, Sandra (Universitat Autònoma de Barcelona. Departament de Química)
Sánchez-Martín, María-Jesús (Universitat Autònoma de Barcelona. Departament de Química)
Amigo, Jose Manuel (Universidad del País Vasco. Departamento de Química Analítica)
Valiente Malmagro, Manuel (Universitat Autònoma de Barcelona. Departament de Química)

Date:	2022
Abstract:	The limitations to assess dental enamel remineralization have been overcome by a methodology resulting from the appropriate combination of synchrotron radiation-based techniques on both, infrared microspectroscopy and micro X-ray diffraction, with the help of specific data mining. Since amelogenin plays a key role in modulating the mineralization of tooth enamel, we propose a controlled ion release for fluorapatite structural ions (Ca 2+, PO 3-, and F -, also including Zn 2+) by using weak acid and weak base ion-exchange resins in the presence of amelogenin to remineralize the surface of etched teeth. This combination provides the necessary ions for enamel remineralization and a guide for crystal growth due to the protein. Remineralized tooth samples were analyzed by applying the indicated methodology. The synchrotron data were treated using principal component analysis and multivariate curve resolution to analyze the mineral layer formed in the presence and absence of amelogenin. The remineralizing treatment created a fluorapatite layer free of carbonate impurities and with a similar orientation to that of the natural enamel thanks to amelogenin contribution.
Grants:	Ministerio de Economía y Competitividad CTM2015-65414-C2-1-R Agència de Gestió d'Ajuts Universitaris i de Recerca FI-2017
Note:	Altres ajuts: acords transformatius de la UAB
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Analytical chemistry, Vol. 94, Issue 13 (April 2022) , p. 5359-5366, ISSN 1520-6882

DOI: 10.1021/acs.analchem.1c05498
PMID: 35319204

8 p, 5.7 MB

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