Biomedical point-of-care microanalyzer for potentiometric determination of ammonium ion in plasma and whole blood
Calvo-Lopez, Antonio (Universitat Autònoma de Barcelona. Departament de Química)
Rebollo Calderón, Beatriz (Universitat Autònoma de Barcelona. Departament de Química)
Ormazábal, Aida (Institut de Recerca Sant Joan de Déu)
Artuch, R. (Institut de Recerca Sant Joan de Déu)
Rosell Ferrer, Javier (Universitat Politècnica de Catalunya. Departament d'Enginyeria Electrònica)
Alonso Chamarro, Julián (Universitat Autònoma de Barcelona. Departament de Química)
Puyol Bosch, M. del Mar (Universitat Autònoma de Barcelona. Departament de Química)

Date:	2022
Abstract:	Some inborn errors of metabolism and other diseases can result in increasing blood ammonium (hyperammonemia episodes), which can cause serious neurological complications in patients or even death. Early diagnosis, follow up and treatment are essential to minimize irreversible damages in brain. Currently, adequate analytical instrumentation for the necessary ammonium bedside determination is not available in all health centers but only in clinical laboratories of reference hospitals. We therefore have developed a low cost and portable potentiometric Point-of-Care microanalyzer (POC) to address this problem. It consists of a cyclic olefin copolymer-based microanalyzer, the size of a credit card and working in continuous flow, which integrates microfluidics, a gas-diffusion module and a potentiometric detection system. The analytical features achieved are a linear range from 30 to 1000 μmol L NH , a detection limit of 18 μmol L NH and a required sample volume of 100 μL, which comply with the medical requirements. Plasma and blood samples are analyzed with no significant differences observed between ammonium concentrations obtained with both the proposed microanalyzer and the reference method. This demonstrates the value of the developed POC for bedside clinical applications.
Grants:	Instituto de Salud Carlos III DTS18/00075 Instituto de Salud Carlos III DTS18/00104 Agencia Estatal de Investigación CTQ2017-85011-R Agencia Estatal de Investigación PID2020-117216RB-I00 Agencia Estatal de Investigación PDC2021-121558-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-220
Note:	Altres ajuts: acords transformatius de la UAB
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Blood ammonium ; Hyperammonemia ; Monitoring ; Point-of-Care analyzer ; Ion selective electrodes
Published in:	Analytica chimica acta, Vol. 1205 (May 2022) , art. 339782, ISSN 1873-4324

DOI: 10.1016/j.aca.2022.339782
PMID: 35414388

8 p, 1.4 MB

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