A new source of representative secondary PET nanoplastics. Obtention, characterization, and hazard evaluation
Villacorta, Aliro (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Rubio Lorente, Laura (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Alaraby, Mohamed (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
López-Mesas, Montserrat (Universitat Autònoma de Barcelona. Departament de Química)
Fuentes-Cebrian, Victor (Universitat Autònoma de Barcelona. Departament de Química)
Moriones, Oscar Hernando (Institut Català de Nanociència i Nanotecnologia)
Marcos Dauder, Ricardo (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Hernández Bonilla, Alba (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Date: |
2022 |
Abstract: |
Micro and nanoplastics (MNPLs) are emergent environmental pollutants requiring urgent information on their potential risks to human health. One of the problems associated with the evaluation of their undesirable effects is the lack of representative samples, matching those resulting from the environmental degradation of plastic wastes. To such end, we propose an easy method to obtain polyethylene terephthalate nanoplastics from water plastic bottles (PET-NPLs) but, in principle, applicable to any other plastic goods sources. An extensive characterization indicates that the proposed process produces uniform samples of PET-NPLs of around 100 nm, as determined by using AF4 and multi-angle and dynamic light scattering methodologies. An important point to be highlighted is that to avoid the metal contamination resulting from methods using metal blades/burrs for milling, trituration, or sanding, we propose to use diamond burrs to produce metal-free samples. To visualize the toxicological profile of the produced PET-NPLs we have evaluated their ability to be internalized by cells, their cytotoxicity, their ability to induce oxidative stress, and induce DNA damage. In this preliminary approach, we have detected their cellular uptake, but without the induction of significant biological effects. Thus, no relevant increases in toxicity, reactive oxygen species (ROS) induction, or DNA damage -as detected with the comet assay- have been observed. The use of representative samples, as produced in this study, will generate relevant data in the discussion about the potential health risks associated with MNPLs exposures. |
Grants: |
European Commission 965196
|
Note: |
Altres ajuts: acords transformatius de la UAB |
Note: |
Altres ajuts: Juan de la Cierva IJC2020-2686I |
Rights: |
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Language: |
Anglès |
Document: |
Article ; recerca ; Versió publicada |
Subject: |
Nanoplastics ;
Polyethylene terephthalate ;
Physicochemical characterization ;
Cell uptake ;
Cytotoxicity |
Published in: |
Journal of hazardous materials, Vol. 439 (October 2022) , art. 129593, ISSN 1873-3336 |
DOI: 10.1016/j.jhazmat.2022.129593
PMID: 35843083
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Record created 2022-07-26, last modified 2024-04-22