A High-Fat Diet Modifies Brain Neurotransmitter Profile and Hippocampal Proteome and Morphology in an IUGR Pig Model
Yeste, Natalia (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Pérez-Valle, Jorge (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Heras-Molina, Ana (Universidad Complutense de Madrid. Departamento de Producción Animal)
Pesántez-Pacheco, José Luis (Escuela de Medicina Veterinaria y Zootecnia. Facultad de Ciencias Agropecuarias. Universidad de Cuenca. Avda. Doce de Octubre)
Porrini, E. (Hospital Universitario de Canarias (La Laguna))
Gonzalez-Bulnes, Antonio (Universidad CEU Cardenal Herrera. Departamento de Producción y Sanidad Animal)
Bassols Teixidó, Anna Maria (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)

Fecha:	2022
Resumen:	Intrauterine Growth Restriction (IUGR) hinders the correct growth of the fetus during pregnancy due to the lack of oxygen or nutrients. The developing fetus gives priority to brain development ("brain sparing"), but the risk exists of neurological and cognitive deficits at short or long term. On the other hand, diets rich in fat exert pernicious effects on brain function. Using a pig model of spontaneous IUGR, we have studied the effect on the adult of a long-term high-fat diet (HFD) on the neurotransmitter profile in several brain areas, and the morphology and the proteome of the hippocampus. Our hypothesis was that animals affected by IUGR (born with low birth weight) would present a different susceptibility to an HFD when they become adults, compared with normal birth-weight animals. Our results indicate that HFD affected the serotoninergic pathway, but it did not provoke relevant changes in the morphology of the hippocampus. Finally, the proteomic analysis revealed that, in some instances, NBW and LBW individuals respond to HFD in different ways. In particular, NBW animals presented changes in oxidative phosphorylation and the extracellular matrix, whereas LBW animals presented differences in RNA splicing, anterograde and retrograde transport and the mTOR pathway.
Ayudas:	Ministerio de Economía y Competitividad AGL2013-48121-C3-R Ministerio de Economía y Competitividad AGL2015-68463-C2-2-P Ministerio de Economía y Competitividad AGL2016-79321-C2-1-R
Nota:	This research was funded by the Spanish Ministry of Economy and Competitivity (AGL2015-68463-C2-2-P to A.B. and projects AGL2013-48121-C3-R and AGL2016-79321-C2-1-R to A.G.-B.), cofunded by FEDER. N.Y. is recipient of a graduate student fellowship from the Spanish FPU National Program Grant (Ministry of Science, Innovation and Universities; number FPU17/01881). A.H.-M. is recipient of a graduate student fellowship from the Spanish FPI National Program Grant (Ministry of Science, Innovation and Universities; number BES-2017-080541).
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Publicado en:	Nutrients, Vol. 14 Núm. 16 (august 2022) , p. 3440, ISSN 2072-6643

DOI: 10.3390/nu14163440
PMID: 36014946

26 p, 3.1 MB

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