A Programmable electrochemical Y-shaped DNA scaffold sensor for the single-step detection of antibodies and proteins in untreated biological fluids
Idili, Andrea (Institut Català de Nanociència i Nanotecnologia)
Bonini, A (Institut Català de Nanociència i Nanotecnologia)
Parolo, Claudio (Institut Català de Nanociència i Nanotecnologia)
Álvarez Diduk, Ruslan (Institut Català de Nanociència i Nanotecnologia)
Di Francesco, Fabio (Università degli Studi di Pisa. Dipartimento di Chimica e Chimica Industriale)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Date:	2022
Abstract:	Proteins and antibodies are key biomarkers for diagnosing and monitoring specific medical conditions. Currently, gold standard techniques used for their quantification require laborious multi-step procedures, involving high costs and slow response times. It is possible to overcome these limitations by exploiting the chemistry and programmability of DNA to design a reagentless electrochemical sensing platform. Specifically, three DNA single strands are engineered that can self-assemble into a Y-shaped DNA nanostructure that resembles one of the IgGs. In order to convert this DNA nanostructure into a responsive DNA-scaffold bioreceptor, it is modified including two recognition elements, two redox tag molecules, and a thiol group. In the absence of the target, the scaffold receptor can efficiently collide with the electrode surface and generate a strong electrochemical signal. The presence of the target induces its bivalent binding, which produces steric hindrance interactions that limit the receptor's collisional activity. In its bound state, the redox tags can therefore approach the surface at a slower rate, leading to a signal decrease that is quantitatively related to the target concentration. The Y-shape DNA scaffold sensor can detect nanomolar concentrations of antibodies and proteins in <15 min with a single-step procedure directly in untreated biological fluids.
Grants:	European Commission 881603 European Commission 795635 European Commission 754510 Ministerio de Ciencia e Innovación CEX2018-000806-S Agencia Estatal de Investigación SEV-2017-0706
Note:	Altres ajuts: ICN2 was funded by the CERCA programme, Generalitat de Catalunya. The authors acknowledge Consejo Superior de Investigaciones Científicas (CSIC) for the project "COVID19-122" granted in the call "Nuevas ayudas extraordinarias a proyectos de investigación en el marco de las medidas urgentes extraordinarias para hacer frente al impacto económico y social del COVID-19 (Ayudas CSIC-COVID-19)".
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Antibody detection ; DNA-scaffolds ; Nature-inspired biosensors ; Protein detection ; Serology ; Square wave voltammetry
Published in:	Advanced functional materials, Vol. 32, issue 37 (Sep. 2022) , art. 2201881, ISSN 1616-3028

DOI: 10.1002/adfm.202201881

12 p, 1.7 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles