The Efficacy and Biopharmaceutical Properties of a Fixed-Dose Combination of Disulfiram and Benzyl Benzoate
Lajarin-Reinares, Maria (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Martinez-Esteve, Elia (Reig Jofre Laboratories)
Pena-Rodríguez, Eloy (Reig Jofre Laboratories)
Cañellas-Santos, Mariona (Reig Jofre Laboratories)
Bulut, Sanja (Bioglan AB)
Karabelas, Kostas (Bioglan AB)
Clauss, Adam (Bioglan AB)
Nieto, Carles (Reig Jofre Laboratories)
Mallandrich, Mireia (Universitat de Barcelona. Departament de Farmàcia i Tecnologia Farmacèutica, i Fisicoquímica)
Fernandez-Campos, Francisco (Reig Jofre Laboratories)

Date:	2022
Abstract:	Scabies and hair lice are parasitic diseases that affect human skin and hair, respectively. The incidence and resistances of these infections are increasing. Tenutex ® (disulfiram and benzyl benzoate emulsion) is an alternative to standard insecticides to avoid resistances. The aim of the work is to evaluate the transdermal absorption and the in vitro efficacy against scabies and hair lice after different exposition times. Dermatomed human skin was used to assess the dermal absorption using a validated High Performance Liquid Chromatography (HPLC) method. HEK001 keratinocytes were used to evaluate the cytotoxicity of benzyl benzoate. Only benzyl benzoate was able to cross the skin, but it did not show cytotoxicity at any of the tested concentrations. The product efficacy was tested on Psoroptes ovis after direct contact and after administration on sheep skin explants at different contact times. Permethrin/malathion-resistant strains of Pediculus humanis capitis adults and eggs were directly exposed to Tenutex, and the vitality and hatchability, respectively, were evaluated. The anti-scabies study demonstrated that exposure for 6 or 24 h completely eradicated the parasite. The pediculicidal activity of Tenutex exhibited superior efficacy than standard treatment on resistant lice. The positive results obtained suggest that Tenutex ® is a good treatment option, especially in drug resistance situations.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Disulfiram ; Benzyl benzoate ; Skin permeation ; Topical medication ; Antiparasitic agents ; Scabies ; Lice
Published in:	International journal of molecular sciences, Vol. 23, Issue 18 (September 2022) , art. 10969, ISSN 1422-0067

DOI: 10.3390/ijms231810969
PMID: 36142878

16 p, 1.6 MB

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