Low-Cost, User-Friendly, All-Integrated Smartphone-Based Microplate Reader for Optical-Based Biological and Chemical Analyses
Bergua Canudo, José Francisco (Institut Català de Nanociència i Nanotecnologia)
Álvarez Diduk, Ruslan (Institut Català de Nanociència i Nanotecnologia)
Idili, Andrea (Institut Català de Nanociència i Nanotecnologia)
Parolo, Claudio (Institut Català de Nanociència i Nanotecnologia)
Maymó, M. (Institut Català de Nanociència i Nanotecnologia)
Hu, Liming (Institut Català de Nanociència i Nanotecnologia)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Date:	2022
Abstract:	The quantitative detection of different molecular targets is of utmost importance for a variety of human activities, ranging from healthcare to environmental studies. Bioanalytical methods have been developed to solve this and to achieve the quantification of multiple targets from small volume samples. Generally, they can be divided into two different classes: point of care (PoC) and laboratory-based approaches. The former is rapid, low-cost, and user-friendly; however, the majority of the tests are semiquantitative, lacking in specificity and sensitivity. On the contrary, laboratory-based approaches provide high sensitivity and specificity, but the bulkiness of experimental instruments and complicated protocols hamper their use in resource-limited settings. In response, here we propose a smartphone-based device able to support laboratory-based optical techniques directly at the point of care. Specifically, we designed and fabricated a portable microplate reader that supports colorimetric, fluorescence, luminescence, and turbidity analyses. To demonstrate the potential of the device, we characterized its analytical performance by detecting a variety of relevant molecular targets (ranging from antibodies, toxins, drugs, and classic fluorophore dyes) and we showed how the estimated results are comparable to those obtained from a commercial microplate reader. Thanks to its low cost (<.
Grants:	Agencia Estatal de Investigación SEV-2017-0706 European Commission 689341 Agencia Estatal de Investigación MAT2017-87202-P881603 European Commission 881603 European Commission 754510
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	American Chemical Society ; Biological analysis ; Chemical analyse ; Low-costs ; Microplate readers ; Molecular targets ; Optical- ; Point of care ; Quantitative detection ; User friendly
Published in:	Analytical chemistry, Vol. 94, Issue 2 (January 2022) , p. 1271-1285, ISSN 1520-6882

DOI: 10.1021/acs.analchem.1c04491

15 p, 9.7 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles