Label-free and reagentless electrochemical genosensor based on graphene acid for meat adulteration detection
Flauzino, J. M. R. (Institut Català de Nanociència i Nanotecnologia)
Nguyen, Emily P. (Institut Català de Nanociència i Nanotecnologia)
Yang, Qiuyue (Institut Català de Nanociència i Nanotecnologia)
Rosati, Giulio (Institut Català de Nanociència i Nanotecnologia)
Panáček, David (Institut Català de Nanociència i Nanotecnologia)
Brito-Madurro, Ana G. (Federal University of Uberlândia. Institute of Biotechnology (Brazil))
Madurro, João M. (Federal University of Uberlândia. Institute of Chemistry (Brazil))
Bakandritsos, Aristides (VŠB-Technical University of Ostrava. Centre of Energy and Environmental Technologies. Nanotechnology Centre (Czech Republic))
Otyepka, Michal (VŠB-Technical University of Ostrava. IT4Innovations (Czech Republic))
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Date:	2022
Abstract:	With the increased demand for beef in emerging markets, the development of quality-control diagnostics that are fast, cheap and easy to handle is essential. Especially where beef must be free from pork residues, due to religious, cultural or allergic reasons, the availability of such diagnostic tools is crucial. In this work, we report a label-free impedimetric genosensor for the sensitive detection of pork residues in meat, by leveraging the biosensing capabilities of graphene acid - a densely and selectively functionalized graphene derivative. A single stranded DNA probe, specific for the pork mitochondrial genome, was immobilized onto carbon screen-printed electrodes modified with graphene acid. It was demonstrated that graphene acid improved the charge transport properties of the electrode, following a simple and rapid electrode modification and detection protocol. Using non-faradaic electrochemical impedance spectroscopy, which does not require any electrochemical indicators or redox pairs, the detection of pork residues in beef was achieved in less than 45 min (including sample preparation), with a limit of detection of 9% w/w pork content in beef samples. Importantly, the sample did not need to be purified or amplified, and the biosensor retained its performance properties unchanged for at least 4 weeks. This set of features places the present pork DNA sensor among the most attractive for further development and commercialization. Furthermore, it paves the way for the development of sensitive and selective point-of-need sensing devices for label-free, fast, simple and reliable monitoring of meat purity.
Grants:	European Commission 881603 Agencia Estatal de Investigación SEV-2017-0706 European Commission 683024
Note:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Food adulteration ; DNA biosensor ; Non-faradaic electrochemical impedance spectroscopy ; Beef ; Pork
Published in:	Biosensors & bioelectronics, Vol. 195 (January 2022) , art. 113628, ISSN 1873-4235

DOI: 10.1016/j.bios.2021.113628

Postprint 29 p, 1.4 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles