Radiation-induced g-H2AX foci in different leukocyte subpopulations
López Vásquez, Juan Sebastián (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Pujol Canadell, Mònica (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Puig, Pedro (Universitat Autònoma de Barcelona. Departament de Matemàtiques)
Armengol Rosell, Gemma (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Barquinero, Joan Francesc (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)

Publicación:	Universitat Autònoma de Barcelona, 2022
Resumen:	To evaluate the γ-H2AX foci in peripheral mononucleated cells (PBMCs) from an exposed person is considered as an early assay for biological dosimetry. However, overdispersion in the γ-H2AX foci cell distribution is generally reported. This make it difficult to use this biomarker to assess non-homogenoeus exposures. The data of this research aims to evaluate: A) the possible differences in the radiosensitivity of the different cell subtypes present in the PBMCs, and B) to evaluate the distribution of γ-H2AX foci per cell in each cell subtype. Peripheral blood samples from three healthy donors were obtained to separate total PBMCs, and CD3+, C4+, CD8+, CD19+, and CD56+ cells. Cells were irradiated at 1 and 2 Gy and incubated at 37 °C for 1, 2, 4, and 24 h. Sham-irradiated cells were also analyzed. γ-H2AX foci were detected by microspcopy after immunofluorescence staining. For each condition, 250 nuclei were considered.
Nota:	Altres ajuts: Spanish Consejo de Seguridad Nuclear (BOE-A-2019-311)
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons en que s'entrega l'obra al domini públic. Podeu copiar, modificar, distribuir l'obra i fer-ne comunicació pública, fins i tot amb finalitat comercial, sense demanar cap mena de permís.
Lengua:	Anglès
Documento:	Conjunt de dades
Materia:	γ-H2AX foci distribution ; Peripheral mononuleated cells ; Ionizing radiation
Obra relacionada:	López JS, Pujol-Canadell M, Puig P, Armengol G, Barquinero JF. "Evaluation of γ-H2AX foci distribution among different peripheral blood mononucleated cell subtypes". Int J Radiat Biol, Vol. 99, issue 10 (March 2023), p. 1550-1558 https://doi.org/10.1080/09553002.2023.2187480

Enllaç a l'article: https://ddd.uab.cat/record/274084
DOI: 10.5565/ddd.uab.cat/268847

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