Extracellular Reactive Oxygen Species (ROS) Production in Fresh Donkey Sperm Exposed to Reductive Stress, Oxidative Stress and NETosis
Yánez-Ortiz, Iván (Universitat de Girona. Departament de Biologia)
Catalán, Jaime (Universitat de Girona. Departament de Biologia)
Mateo-Otero, Yentel (Universitat de Girona. Departament de Biologia)
Dordas-Perpinyà, Marta (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Gacem, Sabrina (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Yeste, Natalia (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Bassols Teixidó, Anna Maria (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Yeste Oliveras, Marc (Universitat de Girona. Departament de Biologia)
Miró, Jordi (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Date:	2021
Abstract:	Jenny shows a large endometrial reaction after semen influx to the uterus with a large amount of polymorphonuclear neutrophils (PMN) migrating into the uterine lumen. PMN act as a sperm selection mechanism through phagocytosis and NETosis (DNA extrudes and, together with proteins, trap spermatozoa). While a reduced percentage of spermatozoa are phagocytosed by PMN, most are found to be attached to neutrophil extracellular traps (NETs). This selection process together with sperm metabolism produces a large amount of reactive oxygen species (ROS) that influence the reproductive success. The present study aimed to determine the extracellular ROS production in both sperm and PMN. With this purpose, (1) donkey sperm were exposed to reductive and oxidative stresses, through adding different concentrations of reduced glutathione (GSH) and hydrogen peroxide (HO), respectively; and (2) PMN were subjected to NETosis in the presence of the whole semen, sperm, seminal plasma (SP) or other activators such as formyl-methionyl-leucyl-phenylalanine (FMLP). Extracellular ROS production (measured as HO levels) was determined with the Amplex ® Red Hydrogen Peroxide/Peroxidase Assay Kit. Donkey sperm showed more resilience to oxidative stress than to the reductive one, and GSH treatments led to greater HO extracellular production. Moreover, not only did SP appear to be the main inducer of NETosis in PMN, but it was also able to maintain the extracellular HO levels produced by sperm and NETosis.
Grants:	Ministerio de Ciencia e Innovación AGL2017-88329-R
Note:	Altres ajuts: Generalitat de Catalunya 2017-SGR-1229
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Reactive oxygen species (ROS) ; Reduced glutathione (GSH) ; Hydrogen peroxide (HO) ; Polymorphonuclear neutrophils (PMN) ; NETosis ; Seminal plasma (SP) ; Donkey
Published in:	Antioxidants, Vol. 10 (august 2021) , ISSN 2076-3921

DOI: 10.3390/antiox10091367
PMID: 34572999

18 p, 2.4 MB

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Articles > Research articles
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