Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica Rice
Marè, Caterina (Research Centre for Genomics and Bioinformatics)
Zampieri, Elisa (Institute for Sustainable Plant Protection)
Cavallaro, Viviana (University of Milan)
Frouin, Julien (Université de Montpellier)
Grenier, Cécile (Université de Montpellier)
Courtois, Brigitte (Université de Montpellier)
Brottier, Laurent (Université de Montpellier)
Tacconi, Gianni (Research Centre for Genomics and Bioinformatics)
Finocchiaro, Franca (Research Centre for Genomics and Bioinformatics)
Serrat, Xavier (Universitat de Barcelona)
Nogués, Salvador (Universitat de Barcelona)
Bundó Barberà, Mireia (Centre de Recerca en Agrigenòmica)
San Segundo, Blanca (Consejo Superior de Investigaciones Científicas (CSIC))
Negrini, Noemi (Università degli Studi di Milano)
Pesenti, Michele (Università degli Studi di Milano)
Sacchi, Gian Attilio (Università degli Studi di Milano)
Gavina, Giacomo (SIS Società Italiana Sementi)
Bovina, Riccardo (SIS Società Italiana Sementi)
Monaco, Stefano (Research Centre for Engineering and Agro-Food Processing)
Tondelli, Alessandro (Research Centre for Genomics and Bioinformatics)
Cattivelli, Luigi (Research Centre for Genomics and Bioinformatics)
Valè, Giampiero (University of Piemonte Orientale)

Fecha:	2023
Resumen:	Rice is one of the most salt sensitive crops at seedling, early vegetative and reproductive stages. Varieties with salinity tolerance at seedling stage promote an efficient growth at early stages in salt affected soils, leading to healthy vegetative growth that protects crop yield. Saltol major QTL confers capacity to young rice plants growing under salt condition by maintaining a low Na + /K + molar ratio in the shoots. Marker-assisted backcross (MABC) procedure was adopted to transfer Saltol locus conferring salt tolerance at seedling stage from donor indica IR64- Saltol to two temperate japonica varieties, Vialone Nano and Onice. Forward and background selections were accomplished using polymorphic KASP markers and a final evaluation of genetic background recovery of the selected lines was conducted using 15,580 SNP markers obtained from Genotyping by Sequencing. Three MABC generations followed by two selfing, allowed the identification of introgression lines achieving a recovery of the recurrent parent (RP) genome up to 100% (based on KASP markers) or 98. 97% (based on GBS). Lines with highest RP genome recovery (RPGR) were evaluated for agronomical-phenological traits in field under non-salinized conditions. VN1, VN4, O1 lines were selected considering the agronomic evaluations and the RPGR% results as the most interesting for commercial exploitation. A physiological characterization was conducted by evaluating salt tolerance under hydroponic conditions. The selected lines showed lower standard evaluation system (SES) scores: 62% of VN4, and 57% of O1 plants reaching SES 3 or SES 5 respectively, while only 40% of Vialone Nano and 25% of Onice plants recorded scores from 3 to 5, respectively. VN1, VN4 and O1 showed a reduced electrolyte leakage values, and limited negative effects on relative water content and shoot/root fresh weight ratio. The Saltol locus was successfully transferred to two elite varieties by MABC in a time frame of three years. The application of background selection until BCF allowed the selection of lines with a RPGR up to 98. 97%. Physiological evaluations for the selected lines indicate an improved salinity tolerance at seedling stage. The results supported the effectiveness of the Saltol locus in temperate japonica and of the MABC procedure for recovering of the RP favorable traits. The online version contains supplementary material available at 10. 1186/s12284-023-00619-2.
Ayudas:	European Commission 678168
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Rice ; Breeding ; MABC ; Salt tolerance ; Saltol ; Background selection ; Recovery percentage ; KASP markers
Publicado en:	Rice, Vol. 16, Num.2 (January 2023) , ISSN 1939-8433

DOI: 10.1186/s12284-023-00619-2
PMID: 36633713

18 p, 2.9 MB

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