A hyaluronic acid demilune scaffold and polypyrrole-coated fibers carrying embedded human neural precursor cells and curcumin for surface capping of spinal cord injuries
Elkhenany, Hoda (Department of Surgery. Alexandria University)
Bonilla, Pablo (Centro de Investigación Príncipe Felipe (València))
Giraldo, Esther (Universitat Politècnica de València. Departament de Biotecnologia)
Alastrue-Agudo, Ana (Centro de Investigación Príncipe Felipe (València))
Edel, Michael J (Universitat Autònoma de Barcelona)
Vicent, María Jesús (Polymer Therapeutics Laboratory)
Gisbert Roca, Fernando (Universitat Politècnica de València)
Marínez Ramos, Cristina (Universitat Politècnica de València)
Rodríguez Doblado, Laura (Universitat Politècnica de València)
Monleón Pradas, Manuel (Universitat Politècnica de València)
Moreno Manzano, Victoria (Neuronal and Tissue Regeneration Laboratory)

Fecha:	2021
Resumen:	Tissue engineering, including cell transplantation and the application of biomaterials and bioactive molecules, represents a promising approach for regeneration following spinal cord injury (SCI). We designed a combinatorial tissue-engineered approach for the minimally invasive treatment of SCI-a hyaluronic acid (HA)-based scaffold containing polypyrrole-coated fibers (PPY) combined with the RAD16-I self-assembling peptide hydrogel (Corning® PuraMatrix™ peptide hydrogel (PM)), human induced neural progenitor cells (iNPCs), and a nanoconjugated form of curcumin (CURC). In vitro cultures demonstrated that PM preserves iNPC viability and the addition of CURC reduces apoptosis and enhances the outgrowth of Nestin-positive neurites from iNPCs, compared to non-embedded iNPCs. The treatment of spinal cord organotypic cultures also demonstrated that CURC enhances cell migration and prompts a neuron-like morphology of embedded iNPCs implanted over the tissue slices. Following sub-acute SCI by traumatic contusion in rats, the implantation of PM-embedded iNPCs and CURC with PPY fibers supported a significant increase in neuro-preservation (as measured by greater βIII-tubulin staining of neuronal fibers) and decrease in the injured area (as measured by the lack of GFAP staining). This combination therapy also restricted platelet-derived growth factor expression, indicating a reduction in fibrotic pericyte invasion. Overall, these findings support PM-embedded iNPCs with CURC placed within an HA demilune scaffold containing PPY fibers as a minimally invasive combination-based alternative to cell transplantation alone.
Ayudas:	Agencia Estatal de Investigación RTI2018-095872-B-C21 Agencia Estatal de Investigación RTI2018-095872-B-C22
Nota:	Altres ajuts: Generalitat de València, H2020-FETOPEN-2018-2019-2020-01
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Publicado en:	Biomedicines, Vol. 9 Núm. 12 (december 2021) , p. 1928, ISSN 2227-9059

DOI: 10.3390/biomedicines9121928
PMID: 34944744

19 p, 9.6 MB

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