Follicular Atresia, Cell Proliferation, and Anti-Mullerian Hormone in Two Neotropical Primates (Aotus nancymae and Sapajus macrocephalus)
Cardoso, Deise de Lima (Universidade Federal Rural da Amazônia. Programa de Pós-Graduação em Saúde e Produção Animal na Amazônia)
Guimarães, Diva Anélie de Araújo (Universidade Federal do Pará. Programa de pós-graduação em Ciência Animal)
Mayor Aparicio, Pedro Ginés (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Ferreira, Maria Auxiliadora Pantoja (Universidade Federal do Pará. Programa de pós-graduação em Ciência Animal)
Coutinho, Leandro Nassar (Universidade Federal do Pará. Programa de pós-graduação em Ciência Animal)
Monteiro, F.O.B (Universidade Federal Rural da Amazônia. Programa de Pós-Graduação em Saúde e Produção Animal na Amazônia)

Date:	2023
Abstract:	Reproductive processes in Neotropical primates are still poorly understood. Complications arising in these processes may result in a compromised reproductive condition for a significant portion of these species. We evaluated the follicular atresia, cell proliferation, and anti-Mullerian hormone presence in two Neotropical primates, and in three sexual phases (follicular, luteal, and gestational). Our results contribute to a greater understanding of the ovary morphofunctional processes that act in follicular selection, recruitment, and degeneration in these species. This study evaluated the follicular atresia, cell proliferation, and anti-Mullerian hormone action in Aotus nancymae and Sapajus macrocephalus during three sexual phases (follicular, luteal, and gestational). Follicular quantification and immunolocalization of Caspase-3 protein, B-cell lymphoma 2 (BCL-2), proliferating cell nuclear antigen (PCNA), and anti-Mullerian hormone (AMH) were performed. A significant difference in the quantification between preantral and antral follicles, with a progressive decrease in the antrals, was identified. Protein and hormonal markers varied significantly between follicle cell types (A. nancymae p = 0. 001; S. macrocephalus, p = 0. 002). Immunostaining in the preantral and antral follicles was present in all sexual phases; for Caspase-3, in granulosa cells, oocytes, and stroma; for BCL-2, in granulosa cells, oocytes, and theca; and for PCNA and AMH, in oocytes and granulosa cells. The immunostaining for Caspase-3 was more expressive in the preantral follicles (follicular phase, p < 0. 05), while that for BCL-2 and PCNA was more expressive in the antral follicles of the follicular phase. The AMH was more expressive in the primary and antral follicles of nonpregnant females, in both the follicular and luteal phases. Our results contribute to understanding the ovarian follicular selection, recruitment, and degeneration of these species.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Owl monkey ; Night monkey ; Capuchin monkey ; Ovary ; Folliculogenesis ; Apoptosis
Published in:	Animals, Vol. 13 (march 2023) , ISSN 2076-2615

DOI: 10.3390/ani13061051
PMID: 36978591

13 p, 2.1 MB

The record appears in these collections:
Articles > Research articles
Articles > Published articles