A novel ratiometric fluorescent approach for the modulation of the ydnamic range of lateral flow immunoassays
Sena-Torralba, Amadeo (Institut Català de Nanociència i Nanotecnologia)
Torné-Morató, Helena (Institut Català de Nanociència i Nanotecnologia)
Parolo, Claudio (Institut Català de Nanociència i Nanotecnologia)
Ranjbar, Saba (Institut Català de Nanociència i Nanotecnologia)
Nejad, Mohammad Amin Farahmand (Institut Català de Nanociència i Nanotecnologia)
Álvarez Diduk, Ruslan (Institut Català de Nanociència i Nanotecnologia)
Idili, Andrea (Institut Català de Nanociència i Nanotecnologia)
Hormozi-Nezhad, M.R. (Sharif University of Technology. Department of Chemistry)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Date:	2022
Abstract:	The majority of lateral flow assays (LFAs) use single-color optical labels to provide a qualitative naked-eye detection, however this detection method displays two important limitations. First, the use of a single-color label makes the LFA prone to results misinterpretation. Second, it does not allow the precise modulation of the sensitivity and dynamic range of the test. To overcome these limitations, a ratiometric approach is developed. In particular, using anti-HIgG functionalized red-fluorescent quantum dots on the conjugate pad (as target dependent labels) and blue-fluorescent nanoparticles fixed on the test line (as target independent reporters), it is possible to generate a wide color palette (blue, purple, pink, red) on the test line. It is believed that this strategy will facilitate the development of LFAs by easily adjusting their analytical properties to the needs required by the specific application.
Grants:	European Commission 754510 Ministerio de Ciencia e Innovación CEX2018-000806-S Agencia Estatal de Investigación MAT2017-87202-P Ministerio de Economía y Competitividad SEV-2013-0295
Note:	Altres ajuts: S.-T. and H.T.-M. contributed equally to this work. The authors acknowledge financial support from NACANCELL project PCIN-2016-066 (program Euronanomed 2). This work was also funded by the CERCA Program/Generalitat de Catalunya. The ICN2 was funded by the CERCA program/Generalitat de Catalunya.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Fluorescence ; Lateral flow assay ; Quantum dots ; Ratiometry ; Sensitivity
Published in:	Advanced Materials Technologies, Vol. 7, issue 8 (August 2022) , art. 2101450, ISSN 2365-709X

DOI: 10.1002/admt.202101450

9 p, 2.5 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles