Hazard assessment of different-sized polystyrene nanoplastics in hematopoietic human cell lines
Tavakolpournegari, Alireza (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Annangi, Balasubramanyam (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Villacorta, Aliro (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Banaei, Gooya (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Martin, Joan (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Pastor Benito, Susana (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Marcos Dauder, Ricardo (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Hernández Bonilla, Alba (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Date:	2023
Abstract:	The environmental presence of micro/nanoplastics (MNPLs) is an environmental and human health concern. Such MNPLs can result from the physicochemical/biological degradation of plastic goods (secondary MNPLs) or can result from industrial production at that size, for different commercial purposes (primary MNPLs). Independently of their origin, the toxicological profile of MNPLs can be modulated by their size, as well as by the ability of cells/organisms to internalize them. To get more information on these topics we have determined the ability of three different sizes of polystyrene MNPLs (50, 200, and 500 nm) to produce different biological effects in three different human hematopoietic cell lines (Raji-B, THP-1, and TK6). Results show that none of the three sizes was able to induce toxicity (growth ability) in any of the tested cell types. Although transmission electron microscopy and confocal images showed cell internalization in all the cases, their quantification by flow cytometry demonstrated an important uptake by Raji-B and THP-1 cells, in comparison with TK6 cells. For the first ones, the uptake was negatively associated with the size. Interestingly, when the loss of mitochondrial membrane potential was determined, dose-related effects were observed for Raji-B and THP-1 cells, but not for TK6 cells. These effects were observed for the three different sizes. Finally, when oxidative stress induction was evaluated, no clear effects were observed for the different tested combinations. Our conclusion is that size, biological endpoint, and cell type are aspects modulating the toxicological profile of MNPLs.
Grants:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00731 Agencia Estatal de Investigación PID2020-116789-RB-C43 European Commission 965196
Note:	Altres ajuts: acords transformatius de la UAB
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Polystyrene nanoplastic size ; Raji-B/THP-1/TK6 cells ; Uptake ; Oxidative stress ; Mitochondrial membrane potential
Published in:	Chemosphere, Vol. 325 (June 2023) , art. 138360, ISSN 1879-1298

DOI: 10.1016/j.chemosphere.2023.138360
PMID: 36905991

10 p, 9.2 MB

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