A Novel Localization of METTL7A in Bergmann Glial Cells in Human Cerebellum
Vera-Montecinos, América (Institut de Recerca Sant Joan de Déu)
Galiano-Landeira, Jordi (Institut de Recerca Sant Joan de Déu)
Roldan Molina, Mònica (Institut de Recerca Sant Joan de Déu)
Vidal-Domènech, Francisco (Institut de Recerca Sant Joan de Déu)
Claro Izaguirre, Enrique (Universitat Autònoma de Barcelona. Institut de Neurociències)
Ramos, Belén (Universitat de Vic)
Universitat Autònoma de Barcelona

Fecha:	2023
Resumen:	Methyltransferase-like protein 7A (METTL7A) is a member of the METTL family of methyltransferases. Little information is available regarding the cellular expression of METTL7A in the brain. METTL7A is commonly located in the endoplasmic reticulum and to a lesser extent, in the lipid droplets of some cells. Several studies have reported altered protein and RNA levels in different brain areas in schizophrenia. One of these studies found reduced protein levels of METTL7A in the cerebellar cortex in schizophrenia and stress murine models. Since there is limited information in the literature about METTL7A, we characterized its cellular and subcellular localizations in the human cerebellum using immunohistochemical analysis with laser confocal microscopy. Our study reveals a novel METTL7A localization in GFAP-positive cells, with higher expression in the end-feet of the Bergmann glia, which participate in the cerebrospinal fluid-brain parenchyma barrier. Further 3D reconstruction image analysis showed that METTL7A was expressed in the contacts between the Bergmann glia and Purkinje neurons. METTL7A was also detected in lipid droplets in some cells in the white matter. The localization of METTL7A in the human cerebellar glia limitans could suggest a putative role in maintaining the cerebellar parenchyma homeostasis and in the regulation of internal cerebellar circuits by modulating the synaptic activity of Purkinje neurons.
Ayudas:	Instituto de Salud Carlos III PI18/00213 Generalitat de Catalunya 72160426
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	METTL7A ; Schizophrenia ; Postmortem cerebellum ; Bergmann glia ; Glia limitans ; Cerebrospinal fluid-brain barrier
Publicado en:	International journal of molecular sciences, Vol. 24 (may 2023) , ISSN 1422-0067

DOI: 10.3390/ijms24098405
PMID: 37176112

15 p, 9.9 MB

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