Exploring the Relationship between Neutrophil Activation and Different States of Canine L. infantum Infection : Nitroblue Tetrazolium Test and IFN-γ
Blasi Brugué, Carles (Universitat Autònoma de Barcelona. Hospital Clínic Veterinari)
Martínez-Flórez, Icíar (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Baxarias, Marta (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
del Rio-Velasco, Joan (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Solano Gallego, Laia (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Fecha:	2023
Resumen:	This study aimed to understand the role of neutrophils in canine leishmaniosis (CanL) by assessing neutrophil activation and its relationship with different states of Leishmania infantum infection and antibody and IFN-γ production. The results showed that sick dogs in stage I-mild disease had significantly higher neutrophil activation compared to healthy seronegative and seropositive dogs and sick dogs in advanced stages (II, III-IV). Healthy seropositive dogs exhibited higher neutrophil activation compared to all other groups except sick dogs in stage I. Dogs in advanced disease stages (II, III-IV) did not show significant differences in neutrophil activation compared to healthy seronegative dogs. Furthermore, dogs in stage I had significantly higher IFN-γ concentrations compared to healthy seronegative and sick dogs in advanced disease stages. Dogs in stage II showed higher IFN-γ concentrations compared to healthy seronegative dogs, while no significant differences were observed in dogs in stage III-IV. Healthy seropositive dogs had elevated IFN-γ concentrations compared to healthy seronegative dogs and dogs in stage III-IV. These findings indicate that neutrophil activation is predominant in dogs with mild disease and healthy seropositive dogs with an association with potent IFN-γ production. This study aimed to investigate the role of neutrophils in canine leishmaniosis by assessing neutrophil activation and its relationship with different states of L. infantum infection and antibody and IFN-γ production. Dogs were categorized into five groups: healthy-seronegative (n = 25), healthy-seropositive (n = 21), LeishVet-stage I (n = 25), Leishvet-stage II (n = 41), and LeishVet-stage III-IV (n = 16). Results of the nitroblue tetrazolium reduction test (NBT) showed significantly higher neutrophil activation in stage I (median:17. 17, range: [7. 33-31. 50]%) compared to in healthy-seronegative (4. 10 [1. 20-18. 00]%), healthy-seropositive (7. 65 [3. 98-21. 74]%), stage II (6. 50 [1. 50-28. 70]%), and stage III-IV (7. 50 [3. 00-16. 75]%) groups (p < 0. 0001). Healthy-seropositive dogs also displayed higher values than all groups except stage I. Stages II and III-IV did not show significant differences compared to healthy-seronegative. Regarding IFN-γ, stage I dogs had higher concentrations (median:127. 90, range: [0-3998. 00] pg/mL) than healthy-seronegative (0 [0-109. 50] pg/mL) (p = 0. 0002), stage II (9. 00 [0-5086. 00] pg/mL) (p = 0. 045), and stage III-IV (3. 50 [80. 00-548. 80] pg/mL) (p = 0. 02) dogs. Stage II dogs showed increased IFN-γ compared to healthy-seronegative dogs (p = 0. 015), while stage III-IV dogs had no significant differences compared to healthy-seronegative dogs (p = 0. 12). Healthy-seropositive dogs had elevated IFN-γ concentrations compared to healthy-seronegative dogs (p = 0. 001) and dogs in stage III-IV (p = 0. 03). In conclusion, neutrophil activation was higher in dogs with mild disease and healthy-seropositive dogs, and a relationship between neutrophil activation and the production of IFN-γ was found.
Ayudas:	Ministerio de Economía y Competitividad AGL2015-68477
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	NBT ; Dog ; Immunity ; Interferon-gamma ; Neutrophil ; Leishmaniosis ; Oxidative burst ; Oxidative metabolism
Publicado en:	Veterinary Sciences, Vol. 10 (september 2023) , ISSN 2306-7381

DOI: 10.3390/vetsci10090572
PMID: 37756094

20 p, 1.1 MB

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