Correlative radioimaging and mass spectrometry imaging : a powerful combination to study 14C-graphene oxide in vivo biodistribution
Cazier, Hélène (Université Paris Saclay)
Malgorn, Carole (Université Paris Saclay)
Georgin, Dominique (Université Paris Saclay)
Fresneau, Nathalie (Université Paris Saclay)
Beau, Fabrice (Université Paris Saclay)
Kostarelos, Kostas (Institut Català de Nanociència i Nanotecnologia)
Bussy, Cyrill (University of Manchester)
Campidelli, Stéphane (Université Paris Saclay)
Pinault, Mathieu (Université Paris-Saclay)
Mayne-L'Hermite, Martine (Université Paris-Saclay)
Taran, Frédéric (Université Paris Saclay)
Junot, Christophe (Université Paris Saclay)
Fenaille, François (Université Paris Saclay)
Sallustrau, Antoine (Université Paris Saclay)
Colsch, Benoit (Université Paris Saclay)

Fecha:	2023
Resumen:	Research on graphene based nanomaterials has flourished in the last decade due their unique properties and emerging socio-economic impact. In the context of their potential exploitation for biomedical applications, there is a growing need for the development of more efficient imaging techniques to track the fate of these materials. Herein we propose the first correlative imaging approach based on the combination of radioimaging and mass spectrometry imaging for the detection of Graphene Oxide (GO) labelled with carbon-14 in mice. In this study, C-graphene oxide nanoribbons were produced from the oxidative opening of C-carbon nanotubes, and were then intensively sonicated to provide nano-size C-GO flakes. After Intravenous administration in mice, C-GO distribution was quantified by radioimaging performed on tissue slices. On the same slices, MS-imaging provided a highly resolved distribution map of the nanomaterial based on the detection of specific radical anionic carbon clusters ranging from C2˙ to C9˙ with a base peak at m/z 72 (C) and 74 (C) under negative laser desorption ionization mass spectrometry (LDI-MS) conditions. This proof of concept approach synergizes the strength of each technique and could be advantageous in the pre-clinical development of future Graphene-based biomedical applications.
Ayudas:	European Commission 785219
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Biomedical applications ; Carbon 14 ; Graphene oxide nanoribbon ; Graphene oxides ; In-vivo ; Mass-spectrometry imaging ; Nano-size ; Property ; Socio-economic impacts ; Vivo biodistribution ; Animals ; Carbon Radioisotopes ; Graphite ; Mice ; Nanotubes, Carbon ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Tissue Distribution
Publicado en:	Nanoscale, Vol. 15, Issue 11 (March 2023) , p. 5510-5518, ISSN 2040-3372

DOI: 10.1039/d2nr06753f

9 p, 3.8 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > Institut Català de Nanociència i Nanotecnologia (ICN2)
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