Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates
Brown-Jaque, Maryury (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística)
Calero-Cáceres, William (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística)
Espinal, Paula (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Rodríguez-Navarro, Judith (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Miró, Elisenda (Institut d'Investigació Biomèdica Sant Pau)
González-López, Juanjo 1975- (Hospital Universitari Vall d'Hebron)
Cornejo-Sánchez, Thais (Hospital Universitari Vall d'Hebron)
Hurtado, Juan Carlos (Barcelona Institute for Global Health (ISGlobal))
Navarro Risueño, Ferran (Institut d'Investigació Biomèdica Sant Pau)
Muniesa, Maite (Institut d'Investigació Biomèdica Sant Pau)

Date:	2018
Description:	9 pàg.
Abstract:	Phage particles have emerged as elements with the potential to mobilise antibiotic resistance genes (ARGs) in different environments, including the intestinal habitat. This study aimed to determine the occurrence of ARGs in phage particles present in faecal matter and induced from strains isolated from faeces. Nine ARGs (blaTEM, blaCTX-M-1 group, blaCTX-M-9 group, blaOXA-48, qnrA, qnrS, mecA, sul1 and armA) were quantified by qPCR in the phage DNA fractions of 150 faecal samples obtained from healthy individuals who had not received antibiotic treatment or travelled abroad in the 3 months prior to sample collection. On the suspicion that the detected particles originated from bacterial flora, 82 Escherichia coli and Klebsiella pneumoniae isolates possessing at least one identified ARG (blaTEM, blaCTX-M-1 group, blaCTX-M-9 group, armA, qnrA, qnrS and sul1) were isolated and their capacity to produce phage particles carrying these ARGs following induction was evaluated. Of 150 samples, 72. 7% were positive for at least one ARG, with blaTEM and blaCTX-M-9 group being the most prevalent and abundant. Of the 82 isolates, 51 (62%) showed an increase in the number of copies of the respective ARG in the phage fraction following induction, with blaTEM, blaCTX-M-1 group, blaCTX-M-9 group and sul1 being the most abundant. Phages induced from the isolates were further purified and visualised using microscopy and their DNA showed ARG levels of up to 1010 gene copies/mL. This study highlights the abundance of phage particles harbouring ARGs and indicates that bacterial strains in the intestinal habitat could be source of these particles.
Grants:	Ministerio de Economía y Competitividad RD12/0015 Ministerio de Economía y Competitividad PI13/00329 Ministerio de Economía y Competitividad CD15/00017 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-0073
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Antibiotic resistance ; Bacteriophage ; Faeces ; Horizontal gene transfer ; Transduction ; SDG 3 - Good Health and Well-being
Published in:	International Journal of Antimicrobial Agents, Vol. 51, Issue 3 (March 2018) , p. 434-442, ISSN 1872-7913

DOI: 10.1016/j.ijantimicag.2017.11.014
PMID: 29180282

Postprint 26 p, 358.3 KB

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