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Página principal > Artículos > Artículos publicados > Polyoxometalate-Decorated Gold Nanoparticles Inhibit β-Amyloid Aggregation and Cross the Blood-Brain Barrier in a µphysiological Model |
Fecha: | 2023 |
Resumen: | Alzheimer's disease is characterized by a combination of several neuropathological hallmarks, such as extracellular aggregates of beta amyloid (Aβ). Numerous alternatives have been studied for inhibiting Aβ aggregation but, at this time, there are no effective treatments available. Here, we developed the tri-component nanohybrid system AuNPs@POM@PEG based on gold nanoparticles (AuNPs) covered with polyoxometalates (POMs) and polyethylene glycol (PEG). In this work, AuNPs@POM@PEG demonstrated the inhibition of the formation of amyloid fibrils, showing a 75% decrease in Aβ aggregation in vitro. As it is a potential candidate for the treatment of Alzheimer's disease, we evaluated the cytotoxicity of AuNPs@POM@PEG and its ability to cross the blood-brain barrier (BBB). We achieved a stable nanosystem that is non-cytotoxic below 2. 5 nM to human neurovascular cells. The brain permeability of AuNPs@POM@PEG was analyzed in an in vitro microphysiological model of the BBB (BBB-on-a-chip), containing 3D human neurovascular cell co-cultures and microfluidics. The results show that AuNPs@POM@PEG was able to cross the brain endothelial barrier in the chip and demonstrated that POM does not affect the barrier integrity, giving the green light to further studies into this system as a nanotherapeutic. |
Ayudas: | Agencia Estatal de Investigación RTI2018-097038-B-C21 Agencia Estatal de Investigación RTI2018-097038-B-C22 Agencia Estatal de Investigación PDC2022-133918-C21 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1079 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-015452 Agencia Estatal de Investigación PRE2019-088286 Agencia Estatal de Investigación PLEC2022-009401 European Commission 801370 |
Derechos: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Lengua: | Anglès |
Documento: | Article ; recerca ; Versió publicada |
Materia: | Nanovehicle ; Gold nanoparticles ; Polyoxometalates ; Β-amyloid ; Blood-brain barrier organ-on-a-chip |
Publicado en: | Nanomaterials, Vol. 13, Issue 19 (October 2023) , art. 2697, ISSN 2079-4991 |
18 p, 1.0 MB |