The release of polylactic acid nanoplastics (PLA-NPLs) from commercial teabags : Obtention, characterization, and hazard effects of true-to-life PLA-NPLs
Banaei, Gooya (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
García-Rodríguez, Alba (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Tavakolpournegari, Alireza (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Martín-Pérez, Juan (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Villacorta, Aliro (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Marcos Dauder, Ricardo (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Hernández Bonilla, Alba (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Fecha:	2023
Resumen:	This study investigates MNPLs release from commercially available teabags and their effects on both undifferentiated monocultures of Caco-2 and HT29 and in the in vitro model of the intestinal Caco-2/HT29 barrier. Teabags were subjected to mechanical and thermodynamic forces simulating the preparation of a cup of tea. The obtained dispersions were characterized using TEM, SEM, DLS, LDV, NTA, and FTIR. Results confirmed that particles were in the nano-range, constituted by polylactic acid (PLA-NPLs), and about one million of PLA-NPLs per teabag were quantified. PLA-NPLs internalization, cytotoxicity, intracellular reactive oxygen species induction, as well as structural and functional changes in the barrier were assessed. Results show that PLA-NPLs present high uptake rates, especially in mucus-secretor cells, and bio-persisted in the tissue after 72 h of exposure. Although no significant cytotoxicity was observed after the exposure to 100 µg/mL PLA-NPLs during 48 h, a slight barrier disruption could be detected at short-time periods. The present work reveals new insights into the safety of polymer-based teabags, the behavior of true-to-life MNPLs in the human body, as well as new questions on how repeated and prolonged exposures could affect the structure and function of the human intestinal epithelium.
Ayudas:	European Commission 801370 European Commission 965196 Agencia Estatal de Investigación PID2020-116789 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00731
Nota:	Altres ajuts: acords transformatius de la UAB
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Food contaminants ; Gastrointestinal tract ; Nanoplastics ; Polylactic acid ; Teabags
Publicado en:	Journal of hazardous materials, Vol. 458 (September 2023) , art. 131899, ISSN 1873-3336

DOI: 10.1016/j.jhazmat.2023.131899
PMID: 37354720

12 p, 7.1 MB

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