Implications of protein interaction in the speciation of potential VIVO-pyridinone drugs
Ferraro, Giarita (Università degli Studi di Napoli Federico II. Dipartimento di Scienze Chimiche)
Paolillo, Maddalena (Università degli Studi di Napoli Federico II. Dipartimento di Scienze Chimiche)
Sciortino, Giuseppe (Institut d'Investigacions Químiques de Catalunya)
Pisanu, Federico (Universitàdi Sassari. Dipartimento di Medicina, Chirurgia e Farmacia)
Garribba, Eugenio (Università di Sassari. Dipartimento di Medicina, Chirurgia e Farmacia)
Merlino, Antonello (Università degli Studi di Napoli Federico II. Dipartimento di Scienze Chimiche)

Date:	2023
Description:	11 pàg.
Abstract:	Vanadium complexes (VCs) are promising agents for the treatment, among others, of diabetes and cancer. The development of vanadium-based drugs is mainly limited by a scarce knowledge of the active species in the target organs, which is often determined by the interaction of VCs with biological macromolecules like proteins. Here, we have studied the binding of [VIVO(empp)2] (where Hempp is 1-methyl-2-ethyl-3-hydroxy-4(1H)-pyridinone), an antidiabetic and anticancer VC, with the model protein hen egg white lysozyme (HEWL) by electrospray ionization-mass spectrometry (ESI-MS), electron paramagnetic resonance (EPR), and X-ray crystallography. ESI-MS and EPR techniques reveal that, in aqueous solution, both the species [VIVO(empp)2] and [VIVO(empp)(H2O)]+, derived from the first one upon the loss of a empp(-) ligand, interact with HEWL. Crystallographic data, collected under different experimental conditions, show covalent binding of [VIVO(empp)(H2O)]+ to the side chain of Asp48, and noncovalent binding of cis-[VIVO(empp)2(H2O)], [VIVO(empp)(H2O)]+, [VIVO(empp)(H2O)2]+, and of an unusual trinuclear oxidovanadium(V) complex, [VV3O6(empp)3(H2O)], with accessible sites on the protein surface. The possibility of covalent and noncovalent binding with different strength and of interaction with various sites favor the formation of adducts with the multiple binding of vanadium moieties, allowing the transport in blood and cellular fluids of more than one metal-containing species with a possible amplification of the biological effects.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	SDG 3 - Good Health and Well-being
Published in:	Inorganic chemistry, Vol. 62, issue 21 (May 2023) , p. 8407-8417, ISSN 1520-510X

DOI: 10.1021/acs.inorgchem.3c01041
PMID: 37195003

11 p, 5.5 MB

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