FISH on sperm : spot-counting to stop counting? Not yet
Molina, Òscar (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Sarrate Navas, Zaida (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Vidal, Francesca (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Blanco, Joan (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)

Date:	2009
Abstract:	Objective: To evaluate the reliability and applicability of the spot-counting system (Cytovision Spot AX workstation) which offers an alternative to the tedious manual analysis of sperm fluorescence in situ hybridization (FISH). Design: Manual and automatic analyses were performed and compared. Setting: Universitat Autònoma de Barcelona. Patient(s): Twenty-four men who asked for information on infertility showing different seminal parameters. Intervention(s): A semen sample for each patient was collected and prepared for FISH. Main Outcome Measure(s): A dual-color FISH using specific probes for chromosomes 13 and 21 and a triple-color FISH with centromeric probes for chromosomes 18, X, and Y were used (Vysis). Standard FISH analysis was carried out. Automatic analysis was subsequently performed using a Spot AX system. Result(s): Overall, we performed 120 comparisons. In 116 out of 120 (96. 67%), the percentage of anomalies reported using manual counting fell within the incidence detected using the automatic system. In the remaining comparisons, statistical differences were detected (4 out of 120; 3. 33%). Time consumed by the automatic analysis was always higher than the manual one, being influenced by the characteristics of the preparations. Conclusion(s): The spot-counting system has potential, but before the service is ready to be offered, we still need to overcome some limitations associated with it.
Grants:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2005SGR-00437
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Automatic scoring system ; FISH ; Male infertility ; Spermatozoa
Published in:	Fertility and Sterility, Vol. 92 N.4 (Oct. 2009) , p. 1474-1480, ISSN 1556-5653

DOI: 10.1016/j.fertnstert.2008.07.1779

26 p, 918.0 KB

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