Reusable Colorimetric Biosensors on Sustainable Silk-Based Platforms
Márquez, Augusto (Instituto de Microelectrónica de Barcelona)
Santiago, Sara (Universitat Autònoma de Barcelona. Departament de Química)
Santos, Molíria V. (University of São Paulo. São Carlos Institute of Physics)
Aznar-Cervantes, Salvador (Instituto Murciano de Investigación y Desarrollo Agrario y Ambiental. Departamento de Biotecnología)
Domínguez, Carlos (Instituto de Microelectrónica de Barcelona)
Omenetto, Fiorenzo (Tufts University)
Guirado López, Gonzalo (Universitat Autònoma de Barcelona. Departament de Química)
Muñoz Berbel, Xavier (Instituto de Salud Carlos III)

Fecha:	2023
Resumen:	In biosensor development, silk fibroin is advantageous for providing transparent, flexible, chemically/mechanically stable, biocompatible, and sustainable substrates, where the biorecognition element remains functional for long time periods. These properties are employed here in the production of point-of-care biosensors for resource-limited regions, which are able to display glucose levels without the need for external instrumentation. These biosensors are produced by photopatterning silk films doped with the enzymes glucose oxidase and peroxidase and photoelectrochromic molecules from the dithienylethene family acting as colorimetric mediators of the enzymatic reaction. The photopatterning results from the photoisomerization of dithienylethene molecules in the silk film from its initial uncolored opened form to its pink closed one. The photoisomerization is dose-dependent, and colored patterns with increasing color intensities are obtained by increasing either the irradiation time or the light intensity. In the presence of glucose, the enzymatic cascade reaction is activated, and peroxidase selectively returns closed dithienylethene molecules to their initial uncolored state. Color disappearance in the silk film is proportional to glucose concentration and used to distinguish between hypoglycemic (below 4 mM), normoglycemic (4-6 mM), and hyperglycemic levels (above 6 mM) by visual inspection. After the measurement, the biosensor can be regenerated by irradiation with UV light, enabling up to five measurement cycles. The coupling of peroxidase activity to other oxidoreductases opens the possibility to produce long-life reusable smart biosensors for other analytes such as lactate, cholesterol, or ethanol.
Ayudas:	Agencia Estatal de Investigación PID2019-106171RB-I00 Agencia Estatal de Investigación PID2022-1412930B-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00052 Agencia Estatal de Investigación PID2021-127653NB-C21 Agencia Estatal de Investigación RTI2018-100773-B-C31 Ministerio de Economía y Competitividad TEC2014-54449-C3-1-R Ministerio de Economía y Competitividad BES-2015-072946
Nota:	Altres ajuts: acords transformatius de la UAB
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Silk fibroin ; Colorimetric biosensor ; Photoelectrochromic ; Dithienylethene mediators ; Photopatterning ; Point of care ; Glucose detection
Publicado en:	ACS applied bio materials, Vol. 7, Issue 2 (February 2023) , p. 853-862, ISSN 2576-6422

DOI: 10.1021/acsabm.3c00872
PMID: 38270977

10 p, 7.8 MB

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