Effects of the breed, sex and age on cellular content and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel
Giraldo, Carlos E. (Universidad de Caldas. Departamento de Salud Animal (Manizales, Colòmbia))
López, Catalina (Universidad de Caldas. Departamento de Salud Animal (Manizales, Colòmbia))
Álvarez, María E. (Universidad de Caldas. Departamento de Salud Animal (Manizales, Colòmbia))
Samudio, Ismael J. (Pontificia Universidad Javeriana. Departamento de Nutrición y Bioquímica)
Prades, Marta (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Carmona Ramírez, Jorge Uriel (Universidad de Caldas (Manizales, Colòmbia). Departamento de Salud Animal)

Fecha:	2013
Resumen:	Background: There is no information on the effects of the breed, gender and age on the cellular content and growth factor (GF) release from equine pure-platelet rich plasma (P-PRP) and pure-platelet rich gel (P-PRG). The objectives of this study were: 1) to compare the cellular composition of P-PRP with whole blood and platelet poor plasma (PPP); 2) to compare the concentration of transforming GF beta 1 (TGF-β1) and platelet derived GF isoform BB (PDGF-BB) between P-PRP treated with non-ionic detergent (P-PRP+NID), P-PRG (activated with calcium gluconate -CG-), PPP+NID, PPP gel (PPG), and plasma and; 3) to evaluate and to correlate the effect of the breed, gender and age on the cellular and GF concentration for each blood component. Forty adult horses, 20 Argentinean Creole Horses (ACH) and, 20 Colombian Creole Horses (CCH) were included. Data were analyzed by parametric (i. e. : t-test, one way ANOVA) and non parametric (Kruskal-Wallis test, Wilcoxon test) tests. Correlation analysis was also performed by using the Spearman and Pearson tests. A p ≤ 0. 05 was set as significant for all tests. All the blood components were compared for platelet (PLT), leukocyte (WBC), TGF-β1 and PDGF-BB concentrations. The effect of the breed, gender and age on these variables was analyzed. A P ≤ 0. 05 was accepted as significant for all the tests. Results: PLT counts were 1. 8 and 0. 6 times higher in P-PRP than in whole blood and PPP, respectively; WBC counts were 0. 5 and 0. 1 times lower in P-PRP, in comparison with whole blood and PPP, respectively. TGF-β1 and PDGF-BB concentrations were 2. 3 and 262 times higher, respectively, in P-PRG than in plasma, and 0. 59 and 0. 48 times higher, respectively, in P-PRG than in PPG. P-PRG derived from CCH females or young horses presented significantly (P < 0. 001) higher PDGF-BB concentrations than P-PRG derived from ACH males or older horses. Conclusions: Our results indicated that P-PRP obtained by a manual method was affected by intrinsic factors such as the breed, gender and age. Equine practitioners should be aware that cellular and GF release from P-PRP/P-PRG could change according with the intrinsic variables associated with a patient in particular.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Horse ; Platelet concentrate ; Transforming growth factor beta 1 ; Platelet derived growth factor isoform BB ; Regenerative therapy
Publicado en:	BMC veterinary research, Vol. 9, Núm. 29 (February 2013) , p. 1-10, ISSN 1746-6148

DOI: 10.1186/1746-6148-9-29
PMID: 23402541

10 p, 398.5 KB

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