Expression of GM-CSF receptor in ovine spermatozoa
Rodríguez Gil, Joan Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Silvers, Gabriela (Universidad Austral de Chile)
Flores Valdepeñas, Eva María (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Palomo Peiró, María Jesús (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Ramírez-Reveco, Alfredo (Universidad Austral de Chile)
Rivera del Álamo, Maria Montserrat (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Castro, Maïté Angeles (Universidad Austral de Chile)
Brito, Mónica (Universidad Austral de Chile)
Correa, Jorge E. (Universidad Austral de Chile)
Concha, Ilona I. (Universidad Austral de Chile)

Fecha:	2006
Resumen:	The granulocyte-macrophage colony stimulating factor (GM-CSF) is a pleiotropic cytokine capable of stimulating proliferation, maturation and function of haematopoietic cells. Receptors for this cytokine are composed of two subunits, alpha and beta, and are expressed in myeloid progenitors and mature mononuclear phagocytes, monocytes, eosinophils and neutrophils, as well as in other non-haematopoietic cells. We have previously demonstrated that bull spermatozoa express functional GM-CSF receptors that signal for increased glucose and vitamin-C uptake and enhance several parameters of sperm motility in the presence of glucose or fructose substrates. In this study, we have analyzed the expression of GM-CSF receptors in ovine spermatozoa and studied the effect of GM-CSF on sperm viability and motility after the freezing-thawing process. Immunolocalization and immunoblotting analyses demonstrated that ovine spermatozoa (Xisqueta race) expressed GM-CSF receptors. In addition, GM-CSF partially counteracted the impairing action of freezing/thawing on the percentage of total motility, as well as on the specific motility patterns of each of the separate, motile sperm subpopulations of ram ejaculates subjected to this protocol. These results suggest that GM-CSF can play a role in the resistance of ram spermatozoa to environmental thermal stress.
Ayudas:	Ministerio de Ciencia e Innovación AGL2004-04756-C02/02/GAN
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió acceptada per publicar
Materia:	Spermatozoa ; Sheep
Publicado en:	Reproduction in domestic animals (1990), Vol. 41 (2006) , p. 115-115, ISSN 0936-6768

DOI: 10.1016/j.theriogenology.2007.02.008

13 p, 434.2 KB

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