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A Multi-Antigenic Adenoviral-Vectored Vaccine Improves BCG-Induced Protection of Goats against Pulmonary Tuberculosis Infection and Prevents Disease Progression
Pérez del Val, Bernat (Fundació Centre de Recerca en Sanitat Animal)
Moll Sánchez, Xavier (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Vidal Barba, Enric (Universitat Autònoma de Barcelona. Fundació Centre de Recerca en Sanitat Animal)
Villarreal Ramos, Bernardo (Animal Health and Veterinary Laboratories Agency. TB Research Group (Addlestone, Regne Unit))
Gilbert, Sarah C. (University of Oxford. The Jenner Institute)
Andaluz Martínez, Anna (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Martín, Maite (Fundació Centre de Recerca en Sanitat Animal)
Nofrarías Espadamala, Miquel (Fundació Centre de Recerca en Sanitat Animal)
McShane, Helen (University of Oxford. The Jenner Institute)
Vordermeier, H. Martin (Animal Health and Veterinary Laboratories Agency. TB Research Group (Addlestone, Regne Unit))
Domingo, Mariano (Fundació Centre de Recerca en Sanitat Animal)

Date: 2013
Abstract: The "One world, one health" initiative emphasizes the need for new strategies to control human and animal tuberculosis (TB) based on their shared interface. A good example would be the development of novel universal vaccines against Mycobacterium tuberculosis complex (MTBC) infection. This study uses the goat model, a natural TB host, to assess the protective effectiveness of a new vaccine candidate in combination with Bacillus Calmette-Guerin (BCG) vaccine. Thirty-three goat kids were divided in three groups: Group 1) vaccinated with BCG (week 0), Group 2) vaccinated with BCG and boosted 8 weeks later with a recombinant adenovirus expressing the MTBC antigens Ag85A, TB10. 4, TB9. 8 and Acr2 (AdTBF), and Group 3) unvaccinated controls. Later on, an endobronchial challenge with a low dose of M. caprae was performed (week 15). After necropsy (week 28), the pulmonary gross pathology was quantified using high resolution Computed Tomography. Small granulomatous pulmonary lesions (< 0. 5 cm diameter) were also evaluated through a comprehensive qualitative histopathological analysis. M. caprae CFU were counted from pulmonary lymph nodes. The AdTBF improved the effects of BCG reducing gross lesion volume and bacterial load, as well as increasing weight gain. The number of Ag85A-specific gamma interferon-producing memory T-cells was identified as a predictor of vaccine efficacy. Specific cellular and humoral responses were measured throughout the 13-week post-challenge period, and correlated with the severity of lesions. Unvaccinated goats exhibited the typical pathological features of active TB in humans and domestic ruminants, while vaccinated goats showed only very small lesions. The data presented in this study indicate that multi-antigenic adenoviral vectored vaccines boosts protection conferred by vaccination with BCG.
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: Goats ; Granulomas ; Tuberculosis ; Vaccines ; Enzyme-linked immunoassays ; Bovine tuberculosis in humans ; Necrosis ; Mycobacterium tuberculosis
Published in: PloS one, Vol. 8 Issue 11 (November 2013) , p. e81317, ISSN 1932-6203

DOI: 10.1371/journal.pone.0081317
PMID: 23251417

12 p, 2.0 MB

The record appears in these collections:
Articles > Research articles
Articles > Published articles

 Record created 2015-10-19, last modified 2019-11-07

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