Home > Articles > Published articles > A Simple Technique Based on a Single Optical Trap for the Determination of Bacterial Swimming Pattern |
Date: | 2013 |
Abstract: | Bacterial motility is associated to a wide range of biological processes and it plays a key role in the virulence of many pathogens. Here we describe a method to distinguish the dynamic properties of bacteria by analyzing the statistical functions derived from the trajectories of a bacterium trapped by a single optical beam. The approach is based on the model of the rotation of a solid optically trapped sphere. The technique is easily implemented in a biological laboratory, since with only a small number of optical and electronic components a simple biological microscope can be converted into the required analyzer. To illustrate the functionality of this method, we probed several serovar Typhimurium mutants that differed from the wild-type with respect to their swimming patterns. In a further application, the motility dynamics of the Typhimurium mutant were characterized. |
Grants: | Ministerio de Economía y Competitividad FIS2008-00114 Ministerio de Economía y Competitividad FIS2011-24409 Ministerio de Economía y Competitividad BFU2011-23478 Agència de Gestió d'Ajuts Universitaris i de Recerca 2009/SGR-1106 |
Rights: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Language: | Anglès |
Document: | Article ; recerca ; Versió publicada |
Subject: | Flagellar rotation ; Flagella ; Bacterial genetics ; Swimming ; Pathogen motility ; Chemotaxis ; Oxygen ; Cell motility |
Published in: | PloS one, Vol. 8 Issue 4 (April 2013) , p. e61630, ISSN 1932-6203 |
10 p, 1.2 MB |