Label free aptasensor for lysozyme detection : a comparison of the analytical performance of two aptamers
Ocaña Tejada, Cristina (Universitat Autònoma de Barcelona. Departament de Química)
Hayat, Akhtar (Universite de Perpignan. Biocapteurs-Analyses-Environnement)
Mishra, Rupesh K. (Universite de Perpignan. Biocapteurs-Analyses-Environnement)
Vasilescu, Alina (International Centre of Biodynamics)
Valle Zafra, Manuel del (Universitat Autònoma de Barcelona. Departament de Química)
Marty, Jean-Louis (Universite de Perpignan. Biocapteurs-Analyses-Environnement)

Fecha:	2015
Resumen:	This work presents a comparison of two different aptamers (COX and TRAN) for the detection of the ubiquitous protein lysozyme using aptamer-based biosensors. The detection is based on the specific recognition by the aptamer immobilized on screen printed carbon electrodes (SPCEs) via diazonium coupling reaction. The quantitative detection of lysozyme protein was achieved by electrochemical impedance spectroscopy (EIS). Very good linear ranges and detection limits for the lysozyme detection were obtained, from 0. 025 to 1 μM and 725nM using aptamer COX and from 0. 025 to 1 μM and 31. 7nM using aptamer TRAN. The obtained results showed that the developed aptasensors exhibit good specificity, stability and reproducibility for lysozyme detection. The aptasensors were also tested in wine samples; very good recovery rates were obtained in the range from 96. 4 to 102% for lysozyme detection. The recovery rates confirm the reliability and suitability of the developed method in wine matrix. The developed method could be a useful and promising platform for detection of lysozyme in different applications.
Ayudas:	Ministerio de Ciencia e Innovación FPI-2011
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió acceptada per publicar
Materia:	Disposable aptasensor ; Lysozyme ; Diazonium chemistry ; Label free detection ; Wine samples
Publicado en:	Bioelectrochemistry, Vol. 105 (October 2015) , p. 72-77, ISSN 1878-562X

DOI: 10.1016/j.bioelechem.2015.05.009

Post-print 23 p, 1.7 MB

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