Web of Science: 6 citas, Scopus: 9 citas, Google Scholar: citas,
Obese dogs with and without obesityrelated metabolic dysfunction – a proteomic approach
Tvarijonaviciute, Asta (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animal)
Cerón Madrigal, José Joaquín (Universidad de Murcia. Departamento de Patología Animal)
Torre, Carlos de (Hospital Clínico Universitario Virgen de la Arrixaca (El Palmar, Múrcia))
Ljubić, Blanka B. (University of Zagreb. Faculty of Veterinary Medicine)
Holden, Shelley L. (University of Liverpool. Department of Obesity and Endocrinology)
Queau, Yann (Royal Canin Research Center)
Morris, Penelope J. (The WALTHAM Centre for Pet Nutrition)
Pastor Milán, Josep (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animal)
German, Alexander J. (University of Liverpool. Department of Obesity and Endocrinology)

Fecha: 2016
Resumen: Background: Approximately 20 % of obese dogs have metabolic disturbances similar to those observed in human metabolic syndrome, a condition known as obesity-related metabolic dysfunction. This condition is associated with insulin resistance and decreased circulating adiponectin concentrations, but clinical consequences have not been reported. In order to define better the metabolic changes associated with obesity-related metabolic dysfunction (ORMD), we compared the plasma proteomes of obese dogs with and without ORMD. A proteomic analysis was conducted on plasma samples from 8 obese male dogs, 4 with ORMD and 4 without ORMD. The samples were first treated for the depletion of high-abundance proteins and subsequently analysed by using 2-DE DIGE methodology. Results: Using mass spectrometry, 12 proteins were identified: albumin, apoliprotein A-I, C2, C3, C5, C4BPA, A2M, Uncharacterised protein (Fragment) OS = Canis familiaris, fibrinogen, IGJ, ITIH2, and glutathione peroxidase. In obese dogs with ORMD, the relative amounts of ten proteins (albumin, apoliprotein A-I, C2, C3, C5, C4BPA, A2M, Uncharacterised protein (Fragment) OS = Canis familiaris, fibrinogen, and ITIH2) were increased and two proteins (IGJ and glutathione peroxidase) were decreased, compared with obese dogs without ORMD. Specific assays were then used to confirm differences in serum albumin, apoliprotein A-I and glutathione peroxidase in a separate group of 20 overweight dogs, 8 with ORMD and 12 without ORMD. Conclusions: The current study provides evidence that, in obese dogs with ORMD, there are changes in expression of proteins involved in lipid metabolism, immune response, and antioxidant status. The clinical significance of these changes remains to be defined.
Derechos: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Lengua: Anglès.
Documento: article ; recerca ; publishedVersion
Materia: Antioxidants ; Complement system ; Dog ; Immune response ; Lipid metabolism ; Metabolic syndrome ; Obesity
Publicado en: BMC Veterinary research, Vol. 12, Num. 212 (September 2016) , p. 1-9, ISSN 1746-6148

DOI: 10.1186/s12917-016-0839-9
PMID: 27646300


9 p, 1.4 MB

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