Web of Science: 10 citas, Scopus: 11 citas, Google Scholar: citas,
Recruitment of a lineage-specific virulence regulatory pathway promotes intracellular infection by a plant pathogen experimentally evolved into a legume symbiont
Capela, Delphine (Institut national de la recherche agronomique (França). Laboratoire des Interactions Plantes Micro-organismes)
Marchetti, Marta (Institut national de la recherche agronomique (França). Laboratoire des Interactions Plantes Micro-organismes)
Clerissi, Camille (Institut national de la recherche agronomique (França). Laboratoire des Interactions Plantes Micro-organismes)
Perrier, Anthony (Institut national de la recherche agronomique (França). Laboratoire des Interactions Plantes Micro-organismes)
Guetta, Dorian (Institut national de la recherche agronomique (França). Laboratoire des Interactions Plantes Micro-organismes)
Gris, Carine (Institut national de la recherche agronomique (França) . Laboratoire des Interactions Plantes Micro-organismes)
Valls, Marc (Centre de Recerca en Agrigenòmica)
Jauneau, Alain (Centre national de la recherche scientifique (França). Fédération de Recherches Agrobiosciences, Interactions, Biodiversity, Plateforme d'Imagerie)
Cruveiller, Stéphane (Centre national de la recherche scientifique (França). Commissariat à l'Energie Atomique et aux Energies Alternatives)
Rocha, Eduardo P.C. (Institut Pasteur (París, França). Microbial Evolutionary Genomics)
Masson-Boivin, Catherine (Institut national de la recherche agronomique (França). Laboratoire des Interactions Plantes Micro-organismes)

Fecha: 2017
Resumen: Ecological transitions between different lifestyles, such as pathogenicity, mutualism and saprophytism, have been very frequent in the course of microbial evolution, and often driven by horizontal gene transfer. Yet, how genomes achieve the ecological transition initiated by the transfer of complex biological traits remains poorly known. Here we used experimental evolution, genomics, transcriptomics and high-resolution phenotyping to analyze the evolution of the plant pathogen Ralstonia solanacearum into legume symbionts, following the transfer of a natural plasmid encoding the essential mutualistic genes. We show that a regulatory pathway of the recipient R. solanacearum genome involved in extracellular infection of natural hosts was reused to improve intracellular symbiosis with the Mimosa pudica legume. Optimization of intracellular infection capacity was gained through mutations affecting two components of a new regulatory pathway, the transcriptional regulator efpR and a region upstream from the RSc0965-0967 genes of unknown functions. Adaptive mutations caused the downregulation of efpR and the over-expression of a downstream regulatory module, the three unknown genes RSc3146-3148, two of which encoding proteins likely associated to the membrane. This over-expression led to important metabolic and transcriptomic changes and a drastic qualitative and quantitative improvement of nodule intracellular infection. In addition, these adaptive mutations decreased the virulence of the original pathogen. The complete efpR/RSc3146-3148 pathway could only be identified in the genomes of the pathogenic R. solanacearum species complex. Our findings illustrate how the rewiring of a genetic network regulating virulence allows a radically different type of symbiotic interaction and contributes to ecological transitions and trade-offs.
Nota: Ajuts: We are grateful to Lidwine Trouilh for helping in NimbleGen microarray hybridizations and Loic Escoriza for mutant construction. J.P.C. and C.C. were supported by the Initiative d'Excellence IDEX UNITI Actions Thématiques Stratégiques program (RHIZOWHEAT 2014) and by the French National Research Agency (ANR-12-ADAP-0014-01). This work was supported by funds from the French National Institute for Agricultural Research (Plant Health and the Environment Division), the French National Research Agency (ANR-12-ADAP-0014-01) and the French Laboratory of Excellence project TULIP (ANR-10-LABX-41). The complete collections of events generated for all the clones from this study are available on the Microscope platform (https://www.genoscope.cns.fr/agc/microscope/expdata/NGSProjectEvo.php, SYMPA tag).
Derechos: Tots els drets reservats
Lengua: Anglès.
Documento: article ; recerca ; acceptedVersion
Materia: Adaptive evolution ; Experimental evolution ; Horizontal gene transfer ; Rhizobium ; Symbiosis ; Pathogenicity
Publicado en: Molecular biology and evolution, Vol. 34, issue 10 (Oct. 2017) , p.2486–2502, ISSN 0737-4038

DOI: 10.1093/molbev/msx165


Post-print
40 p, 3.7 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > CRAG (Centro de Investigación en Agrigenómica)
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 Registro creado el 2017-08-28, última modificación el 2019-07-21



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