The Zn- or Cu-thionein character of a metallothionein determines its metal load when synthesized in physiological (metal-unsupplemented) conditions
Capdevila Vidal, Mercè (Universitat Autònoma de Barcelona. Departament de Química)
Palacios Bonilla, Òscar (Universitat Autònoma de Barcelona. Departament de Química)
Atrian i Ventura, Sílvia (Universitat de Barcelona. Departament de Genètica)

Date: 2010
Abstract: The present work comprises the recombinant synthesis of four metallothioneins (MTs) in metal-unsupplemented cultures and the characterization of the recovered metal complexes by means of analytical and spectrometric techniques. The four MTs are two Drosophila (MtnA and MtnB), one yeast (Crs5), and one mouse (mMT1) metallothionein isoforms. These four MTs exhibit distinct metal binding preferences, from a clear Cu-thionein character to a definite Zn-thionein nature, respectively. Although in all cases, the only metal ion present in the purified complexes is Zn²+, our results highlight an inherently different behaviour of those two types of MTs, in conditions that would mimic their synthesis in physiological environments. Therefore, intrinsically different roles can be hypothesized for the constitutively-produced MT peptides in the absence of any metal overload, depending on their Zn- or Cu-thionein character.
Note: Número d'acord de subvenció MCYT/BIO2009-12513-C02-01
Note: Número d'acord de subvenció MCYT/BIO2009-12513-C02-02
Note: Número d'acord de subvenció AGAUR/2009/SGR-1457
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Published in: Bioinorganic chemistry and applications, Vol. 2010 (2010) , art. 541829, ISSN 1687-479X

PMID: 20467455


6 p, 637.3 KB

The record appears in these collections:
Articles > Research articles
Articles > Published articles

 Record created 2017-12-20, last modified 2018-12-25



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