Direct detection of protein biomarkers in human fluids using site-specific antibody immobilization strategies
Soler Aznar, Maria (Institut Català de Nanociència i Nanotecnologia)
Estévez, M. Carmen (Institut Català de Nanociència i Nanotecnologia)
Alvarez, Mar (Institut Català de Nanociència i Nanotecnologia)
Otte Ortiz, Marinus Albertus (Institut Català de Nanociència i Nanotecnologia)
Sepúlveda, Borja (Institut Català de Nanociència i Nanotecnologia)
Lechuga, Laura M (Institut Català de Nanociència i Nanotecnologia)

Date:	2014
Abstract:	Design of an optimal surface biofunctionalization still remains an important challenge for the application of biosensors in clinical practice and therapeutic follow-up. Optical biosensors offer real-time monitoring and highly sensitive label-free analysis, along with great potential to be transferred to portable devices. When applied in direct immunoassays, their analytical features depend strongly on the antibody immobilization strategy. A strategy for correct immobilization of antibodies based on the use of ProLinker™ has been evaluated and optimized in terms of sensitivity, selectivity, stability and reproducibility. Special effort has been focused on avoiding antibody manipulation, preventing nonspecific adsorption and obtaining a robust biosurface with regeneration capabilities. ProLinker™-based approach has demonstrated to fulfill those crucial requirements and, in combination with PEG-derivative compounds, has shown encouraging results for direct detection in biological fluids, such as pure urine or diluted serum. Furthermore, we have implemented the ProLinker™ strategy to a novel nanoplasmonic-based biosensor resulting in promising advantages for its application in clinical and biomedical diagnosis.
Grants:	Ministerio de Economía y Competitividad TEC2099-08729 Ministerio de Economía y Competitividad OPT-2011-1429-010000
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Antibody immobilization ; ProLinker™ ; Direct immunoassay ; SPR biosensor ; Nanoplasmonic biosensor ; Gold nanodisks ; Clinical diagnosis
Published in:	Sensors (Basel, Switzerland), Vol. 14, Issue 2 (February 2014) , p. 2239-2258, ISSN 1424-8220

DOI: 10.3390/s140202239
PMID: 24481229

20 p, 864.5 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles