Development and validation of a genotype 3 recombinant protein-based immunoassay for hepatitis E virus serology in swine
van der Poel, Wim H. M. (Central Veterinary Institute, Wageningen University and Research Centre (Lelystad, Països Baixos))
Pavio, N.
van der Goot, Jeanet A. (Central Veterinary Institute, Wageningen University and Research Centre (Lelystad, Països Baixos))
van Es, Marieke (Central Veterinary Institute, Wageningen University and Research Centre (Lelystad, Països Baixos))
Martín Castillo, Margarita (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Engel, Bas (Wageningen University and Research Centre (Wageningen, Països Baixos))

Fecha:	2014
Resumen:	Hepatitis E virus (HEV) is classified within the family Hepeviridae, genus Hepevirus. HEV genotype 3 (Gt3) infections are endemic in pigs in Western Europe and in North and South America and cause zoonotic infections in humans. Several serological assays to detect HEV antibodies in pigs have been developed, at first mainly based on HEV genotype 1 (Gt1) antigens. To develop a sensitive HEV Gt3 ELISA, a recombinant baculovirus expression product of HEV Gt3 open reading frame-2 was produced and coated onto polystyrene ELISA plates. After incubation of porcine sera, bound HEV antibodies were detected with anti-porcine anti-IgG and anti-IgM conjugates. For primary estimation of sensitivity and specificity of the assay, sets of sera were used from pigs experimentally infected with HEV Gt3. For further validation of the assay and to set the cutoff value, a batch of 1100 pig sera was used. All pig sera were tested using the developed HEV Gt3 assay and two other serologic assays based on HEV Gt1 antigens. Since there is no gold standard available for HEV antibody testing, further validation and a definite setting of the cutoff of the developed HEV Gt3 assay were performed using a statistical approach based on Bayes' theorem. The developed and validated HEV antibody assay showed effective detection of HEV-specific antibodies. This assay can contribute to an improved detection of HEV antibodies and enable more reliable estimates of the prevalence of HEV Gt3 in swine in different regions.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Hepatitis E virus ; Zoonosis ; Swine ; ELISA
Publicado en:	Brazilian Journal of Medical and Biological Research, Vol. 47 (march 2014) , p. 334-339, ISSN 1414-431X

DOI: 10.1590/1414-431X20133249
PMID: 24676472

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