Simultaneous underexpression of let-7a-5p and let-7f-5p microRNAs in plasma and stool samples from early stage colorectal carcinoma
Ghanbari, Reza (Tehran University of Medical Sciences)
Mosakhani, Neda (The University of Helsinki. Department of Pathology)
Sarhadi, Virinder K. (The University of Helsinki. Department of Pathology)
Armengol Rosell, Gemma (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Nouraee, Nazila (Tarbiat Moddares University. Department of Molecular Genetics)
Mohammadkhani, Ashraf (Tehran University of Medical Sciences)
Khorrami, Samaneh (Tehran University of Medical Sciences)
Arefian, Ehsan (University of Tehran. Department of Microbiology)
Paryan, Mahdi (Pasteur Institute of Iran)
Malekzadeh, Reza (Tehran University of Medical Sciences)
Knuutila, Sakari (The University of Helsinki. Department of Pathology)

Date:	2015
Abstract:	Colorectal cancer (CRC) is the third most common malignancy and the second most common cause of cancer death worldwide. Early detection of CRC can improve patient survival rates; thus, the identification of noninvasive diagnostic markers is urgently needed. MicroRNAs (miRNAs) have extensive potential to diagnose several diseases, including cancer. In this study, we compared the expression pattern of miRNAs from plasma and stool samples of patients with early stages of CRC (I, II) with that of healthy subjects. We performed miRNA profiling using microarrays on plasma and stool samples of eight patients with CRC and four healthy subjects. Seven miRNAs were found to be underexpressed in both plasma and stool samples of patients with CRC versus healthy subjects. Then, we aimed to verify two out of these seven differentially expressed miRNAs (let-7a-5p and let-7f-5p) by quantitative reverse transcriptase polymerase chain reaction on a larger set of plasma and stool samples of 51 patients with CRC and 26 healthy subjects. We confirmed the results of microarray analysis since their expression was significantly lower in stool and plasma samples of patients with CRC. Moreover, receiver operating characteristic curve analysis demonstrated that fecal let-7f expression levels have significant sensitivity and specificity to distinguish between patients with CRC and healthy subjects. In conclusion, if the results are confirmed in larger series of patients, underexpressed let-7a-5p and let-7f-5p miRNAs in both plasma and stool samples of patients with CRC may serve potentially as noninvasive molecular biomarkers for the early detection of CRC.
Note:	The study was supported by Novartis, Sigrid Jusélius Foundation, and Cancer Society of Finland. The authors confirm that the funder had no influence over the study design, content of the article, or selection of this journal.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Mirna ; Let-7 ; Stool DNA ; Plasma DNA ; Early markers ; Colorectal cancer
Published in:	Biomarkers in cancer, Vol. 7, suppl. 1 (2015) , p. 39-48, ISSN 1179-299X

DOI: 10.4137/BIC.S25252
PMID: 26793011
PMID: 26327783

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