Web of Science: 2 citas, Scopus: 2 citas, Google Scholar: citas
MiR858-mediated regulation of flavonoid-specific MYB transcription factor genes controls resistance to pathogen infection in Arabidopsis
Camargo-Ramírez, Rosany (Centre de Recerca en Agrigenòmica)
Val-Torregrosa, Beatriz (Centre de Recerca en Agrigenòmica)
San Segundo, Blanca (Centre de Recerca en Agrigenòmica)

Fecha: 2018
Resumen: MicroRNAs (miRNAs) are a class of short endogenous non-coding small RNAs that direct post-transcriptional gene silencing in eukaryotes. In plants, the expression of a large number of miRNAs has been shown to be regulated during pathogen infection. However, the functional role of the majority of these pathogen-regulated miRNAs has not been elucidated. In this work, we investigated the role of Arabidopsis miR858 in the defense response of Arabidopsis plants to infection by fungal pathogens with necrotrophic (Plectosphaerella cucumerina) or hemibiotrophic (Fusarium oxysporum and Colletotrichum higginsianum) lifestyles. Whereas overexpression of MIR858 enhances susceptibility to pathogen infection, interference with miR858 activity by target mimics (MIM858 plants) results in disease resistance. Upon pathogen challenge, stronger activation of the defense genes PDF1. 2 and PR4 occurs in MIM858 plants than in wild-type plants, whereas pathogen infection induced weaker activation of these genes in MIR858 overexpressor plants. Reduced miR858 activity, and concomitant up-regulation of miR858 target genes, in MIM858 plants, also leads to accumulation of flavonoids in Arabidopsis leaves. The antifungal activity of phenylpropanoid compounds, including flavonoids, is presented. Furthermore, pathogen infection or treatment with fungal elicitors is accompanied by a gradual decrease in MIR858 expression in wild-type plants, suggesting that miR858 plays a role in PAMP (pathogen-associated molecular pattern)-triggered immunity. These data support that miR858 is a negative regulator of Arabidopsis immunity and provide new insights into the relevant role of miR858-mediated regulation of the phenylpropanoid biosynthetic pathway in controlling Arabidopsis immunity.
Nota: Altres ajuts: CERCA Generalitat de Catalunya
Nota: Número d'acord de subvenció MINECO/BES-2016-076289
Nota: Número d'acord de subvenció MINECO/BIO2015-67212-R
Nota: Número d'acord de subvenció MINECO/SEV-2015-0533
Nota: Número d'acord de subvenció MINECO/BIO2012-32838
Nota: Número d'acord de subvenció MECD/FPU12/02812
Derechos: Tots els drets reservats
Lengua: Anglès.
Documento: article ; recerca ; acceptedVersion
Materia: Arabidopsis thaliana ; Colletotrichum higginsianum ; Defense response ; Fusarium oxysporum ; MiR858 ; Plectosphaerella cucumerina
Publicado en: Plant & cell physiology, Vol. 59, issue 1 (Jan. 2018) , p. 190-204, ISSN 0032-0781

DOI: 10.1093/pcp/pcx175


Post-print
29 p, 2.4 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > CRAG (Centro de Investigación en Agrigenómica)
Artículos > Artículos de investigación
Artículos > Artículos publicados

 Registro creado el 2018-02-07, última modificación el 2019-02-19



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