A recombinant Potato virus Y infectious clone tagged with the Rosea1 Visual Marker (PVY-Ros1) facilitates the analysis of viral infectivity and allows the production of large amounts of anthocyanins in plants
Cordero, Teresa (Instituto de Biología Molecular y Celular de Plantas)
Mohamed, Mohamed A. (Instituto de Biología Molecular y Celular de Plantas)
Lopez-Moya, Juan Jose (Centre de Recerca en Agrigenòmica)
Daròs, José-Antonio (Instituto de Biología Molecular y Celular de Plantas)

Fecha:	2017
Resumen:	Potato virus Y (PVY) is a major threat to the cultivation of potato and other solanaceous plants. By inserting a cDNA coding for the Antirrhinum majus Rosea1 transcription factor into a PVY infectious clone, we created a biotechnological tool (PVY-Ros1) that allows infection by this relevant plant virus to be tracked by the naked eye with no need for complex instrumentation. Rosea1 is an MYB-type transcription factor whose expression activates the biosynthesis of anthocyanin pigments in a dose-specific and cell-autonomous manner. Our experiments showed that the mechanical inoculation of solanaceous plants with PVY-Ros1 induced the formation of red infection foci in inoculated tissue and solid dark red pigmentation in systemically infected tissue, which allows disease progression to be easily monitored. By using silver nanoparticles, a nanomaterial with exciting antimicrobial properties, we proved the benefits of PVY-Ros1 to analyze novel antiviral treatments in plants. PVY-Ros1 was also helpful for visually monitoring the virus transmission process by an aphid vector. Most importantly, the anthocyanin analysis of infected tobacco tissues demonstrated that PVY-Ros1 is an excellent biotechnological tool for molecular farming because it induces the accumulation of larger amounts of anthocyanins, antioxidant compounds of nutritional, pharmaceutical and industrial interest, than those that naturally accumulate in some fruits and vegetables well known for their high anthocyanin content. Hence these results support the notion that the virus-mediated expression of regulatory factors and enzymes in plants facilitates easy quick plant metabolism engineering.
Ayudas:	Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Economía y Competitividad BIO2014-54269-R Ministerio de Economía y Competitividad AGL2013-49919-EXP Ministerio de Economía y Competitividad AGL2013-42537-R
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Potato virus Y ; Anthocyanin ; Silver nanoparticles ; Aphid vector ; Molecular farming
Publicado en:	Frontiers in microbiology, Vol. 8 (April 2017) , art. 611, ISSN 1664-302X

DOI: 10.3389/fmicb.2017.00611
PMID: 28428782

11 p, 738.1 KB

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