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A dose-escalation ex vivo study on the effects of intracameral benzalkonium chloride in rabbits
Segarra, Sergi
Leiva, Marta
Costa, Daniel (Fundació Hospital Clínic Veterinari)
Coyo, Natàlia (Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Bellaterra, Spain)
Sabés-Alsina, Maria
Ríos, José
Peña, Teresa (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Fecha: 2018
Resumen: Rabbits are currently not a good model for studying diseases of the corneal endothelium because their corneal endothelial cells (CECs) maintain a high proliferative capacity throughout almost all their life. Addressing this particular feature might allow the use of this species for such a purpose. The aim of this study was to evaluate the corneal endothelial injury after intracameral benzalkonium chloride (BAC) injection into rabbit eyes ex vivo, and to establish the most suitable starting dose for an in vivo study aimed at developing an animal model of corneal endothelial disease. Forty rabbit eyes obtained postmortem by transconjunctival enucleation were divided into 8 groups according to the injected compound: Control (no injection), BSS, and increasing BAC concentrations (0. 005%, 0. 01%, 0. 025%, 0. 05%, 0. 1% and 0. 2%). At 0, 6, 24 and 48 h, ophthalmologic examination of the anterior segment, pachymetry and specular microscopy were performed, and corneas were finally vital-stained and observed under the light microscope to assess the CECs morphology and mortality rate. When compared to BSS, CECs density started to decrease significantly at 0. 025% BAC concentration, while mean cell area, corneal edema and corneal thickness began to increase significantly at 0. 05%, 0. 005% and 0. 1% BAC concentrations, respectively. Concentrations of 0. 05% BAC and above caused significant increases in CECs pleomorphism (decreased hexagonality) and mortality, compared to control and BSS. Ex vivo intracameral BAC injection induces corneal endothelial toxicity in rabbits. However, confirmatory in vivo studies are required to develop the desired model, with 0. 05% BAC being a suggested starting point.
Derechos: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Lengua: Anglès.
Documento: article ; recerca ; publishedVersion
Materia: Endothelial cells ; Corneal endothelium ; Corneal ; Endothelial dystrophy ; Endothelial degeneration
Publicado en: BMC Veterinary Research, Vol. 14 (february 2018) , ISSN 1746-6148

PMID: 29394897
DOI: 10.1186/s12917-018-1349-8

9 p, 983.1 KB

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