Web of Science: 15 citas, Scopus: 16 citas, Google Scholar: citas,
Straightforward Immunosensing Platform Based on Graphene Oxide-Decorated Nanopaper : A Highly Sensitive and Fast Biosensing Approach
Cheeveewattanagul, Nopchulee (Institut Català de Nanociència i Nanotecnologia)
Morales Narváez, Edén (Institut Català de Nanociència i Nanotecnologia)
Hassan, Abdel-Raim H.A. (Institut Català de Nanociència i Nanotecnologia)
Bergua. José Francisco (Institut Català de Nanociència i Nanotecnologia)
Surareungchai, Werasak (King Mongkut's University of Technology Thonburi (Bangkon, Tailàndia))
Somasundrum, Mithran (King Mongkut's University of Technology Thonburi (Bangkon, Tailàndia))
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Fecha: 2017
Resumen: Immunoassays are nowadays a crucial tool for diagnostics and drug development. However, they often involve time-consuming procedures and need at least two antibodies in charge of the capture and detection processes, respectively. This study reports a nanocomposite based on graphene oxide-coated nanopaper (GONAP) facilitating an advantageous immunosensing platform using a single antibody and without the need for washing steps. The hydrophilic, porous, and photoluminescence-quenching character of GONAP allows for the adsorption and quenching of photoluminescent quantum dots nanocrystals complexed with antibodies (Ab-QDs), enabling a ready-to-use immunosensing platform. The photoluminescence is recovered upon immunocomplex (antibody-antigen) formation which embraces a series of interactions (hydrogen bonding, electrostatic, hydrophobic, and Van der Waals interactions) that trigger desorption of the antigen-Ab-QD complex from GONAP surface. However, the antigen is then attached onto the GONAP surface by electrostatic interactions leading to a spacer (greater than ≈20 nm) between Ab-QDs and GONAP and thus hindering nonradiative energy transfer. It is demonstrated that this simple—yet highly sensitive—platform represents a virtually universal immunosensing approach by using small-sized and big-sized targets as model analytes, those are, human-IgG protein and Escherichia coli bacteria. In addition, the assay is proved effective in real matrices analysis, including human serum, poultry meat, and river water. GONAP opens the way to conceptually new paper-based devices for immunosensing, which are amenable to point of care applications and automated diagnostics.
Nota: Número d'acord de subvenció EC/H2020/689341
Nota: Número d'acord de subvenció MINECO/SEV-2013-0295
Nota: Número d'acord de subvenció AGAUR/2014/SGR-260
Nota: Número d'acord de subvenció TRF/PHD/0109/2553
Derechos: Tots els drets reservats
Lengua: Anglès.
Documento: article ; recerca ; acceptedVersion
Materia: Nanocomposites ; Optical biosensors ; Biophotonics ; Paper-based devices ; Immunoassay ; Diagnostics
Publicado en: Advanced Functional Materials, Vol. 27, Núm. 38 (October 2017) , article 1702741, ISSN 1616-301X

DOI: 10.1002/adfm.201702741


Post-print
22 p, 2.9 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > Institut Català de Nanociència i Nanotecnologia (ICN2)
Artículos > Artículos de investigación
Artículos > Artículos publicados

 Registro creado el 2018-04-30, última modificación el 2019-06-13



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