Circulating extracellular vesicles as potential biomarkers in chronic fatigue syndrome/myalgic encephalomyelitis : an exploratory pilot study
Castro-Marrero, Jesús (Hospital Universitari Vall d'Hebron)
Serrano-Pertierra, Esther (Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo)
Oliveira-Rodríguez, Myriam (Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo)
Zaragoza, Maria Cleofé (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Martínez-Martínez, Alba (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Blanco-López, María del Carmen (Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo)
Alegre Martín, José (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Universitat Autònoma de Barcelona

Fecha:	2018
Resumen:	Chronic Fatigue Syndrome (CFS), also known as Myalgic Encephalomyelitis (ME) is an acquired, complex and multisystem condition of unknown etiology, no established diagnostic lab tests and no universally FDA-approved drugs for treatment. CFS/ME is characterised by unexplicable disabling fatigue and is often also associated with numerous core symptoms. A growing body of evidence suggests that extracellular vesicles (EVs) play a role in cell-to-cell communication, and are involved in both physiological and pathological processes. To date, no data on EV biology in CFS/ME are as yet available. The aim of this study was to isolate and characterise blood-derived EVs in CFS/ME. Blood samples were collected from 10 Spanish CFS/ME patients and 5 matched healthy controls (HCs), and EVs were isolated from the serum using a polymer-based method. Their protein cargo, size distribution and concentration were measured by Western blot and nanoparticle tracking analysis. Furthermore, EVs were detected using a lateral flow immunoassay based on biomarkers CD9 and CD63. We found that the amount of EV-enriched fraction was significantly higher in CFS/ME subjects than in HCs (p = 0. 007) and that EVs were significantly smaller in CFS/ME patients (p = 0. 014). Circulating EVs could be an emerging tool for biomedical research in CFS/ME. These findings provide preliminary evidence that blood-derived EVs may distinguish CFS/ME patients from HCs. This will allow offer new opportunities and also may open a new door to identifying novel potential biomarkers and therapeutic approaches for the condition.
Nota:	Altres ajuts: This work was partially supported by the Consejería de Economía y Empleo del Principado de Asturias (Plan de Ciencias, Tecnología e Innovación 2013-2017) under grant number GRUPIN14-022. The authors are gratefully acknowledged for the support from the European Regional Development Fund (ERDF). Plan de Ciencia, Tecnología e Innovación [GRUPIN14-022].
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Chronic fatigue syndrome ; Myalgic encephalomyelitis ; Extracellular vesicles ; Lateral flow immunoassay system ; Tetraspanins
Publicado en:	Journal of extracellular vesicles, Vol. 7 (march 2018) , ISSN 2001-3078

DOI: 10.1080/20013078.2018.1453730
PMID: 29696075

6 p, 1.5 MB

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