Skeletal muscle interleukin-6 regulates metabolic factors in i during and exercise training
Knudsen, Jakob G. (Centre for Inflammation and Metabolism, The August Krogh Centre, University of Copenhagen)
Bertholdt, Lærke (Centre for Inflammation and Metabolism, The August Krogh Centre, University of Copenhagen)
Joensen, Ella (Centre for Inflammation and Metabolism, The August Krogh Centre, University of Copenhagen)
Lassen, Signe B. (Centre for Inflammation and Metabolism, The August Krogh Centre, University of Copenhagen)
Hidalgo Pareja, Juan (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Pilegaard, Henriette (Centre for Inflammation and Metabolism, The August Krogh Centre, University of Copenhagen)

Fecha:	2015
Resumen:	To investigate the role of skeletal muscle (SkM) interleukin (IL)-6 in the regulation of adipose tissue metabolism. Muscle-specific IL-6 knockout (IL-6 MKO) and IL-6 loxP/loxP (Floxed) mice were subjected to standard rodent diet (Chow), high-fat diet (HFD), or HFD in combination with exercise training (HFD ExTr) for 16 weeks. Total fat mass increased (P < 0. 05) in both genotypes with HFD. However, HFD IL-6 MKO mice had lower (P < 0. 05) inguinal adipose tissue (iWAT) mass than HFD Floxed mice. Accordingly, iWAT glucose transporter 4 (GLUT4) protein content, 5'AMP activated protein kinase (AMPK) Thr172 phosphorylation, and fatty acid synthase (FAS) mRNA content were lower (P < 0. 05) in IL-6 MKO than Floxed mice on Chow. In addition, iWAT AMPK Thr172 and hormone-sensitive lipase (HSL) Ser565 phosphorylation as well as perilipin protein content was higher (P < 0. 05) in HFD IL-6 MKO than HFD Floxed mice, and pyruvate dehydrogenase E1α (PDH-E1α) protein content was higher (P < 0. 05) in HFD ExTr IL-6 MKO than HFD ExTr Floxed mice. These findings indicate that SkM IL-6 affects iWAT mass through regulation of glucose uptake capacity as well as lipogenic and lipolytic factors.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Publicado en:	Obesity (Silver Spring, Md.), Vol. 23 (june 2015) , p. 1616-1624, ISSN 1930-739X

DOI: 10.1002/oby.21139
PMID: 26109166

9 p, 525.5 KB

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