Home > Articles > Published articles > An immobilized and highly stabilized self-sufficientmonooxygenase as biocatalyst for oxidative biotransformations |
Date: | 2018 |
Abstract: | Background: the requirement for expensive cofactors that must be efficiently recycled is one of the major factors hindering the wide implementation of industrial biocatalytic oxidation processes. In this research, a sustainable approach based on immobilized self-sufficient Baeyer-Villiger monooxygenases is discussed. - Results: a bifunctional biocatalyst composed of an NADPH-dependent cyclohexanone monooxygenase (CHMO) fused to an NADP+-accepting phosphite dehydrogenase (PTDH) catalyzes ϵ-caprolactone synthesis from cyclohexanone, using phosphite as a cheap sacrificial substrate for cofactor regeneration. Several immobilized derivatives of the fused enzyme have been prepared with high immobilization yield (97%); the one obtained by affinity adsorption on Co-IDA (Co: cobalt chelated; IDA: iminodiacetic acid) support has shown to be highly stable affording average yields of 80% after 18 reaction cycles. - Conclusions -the immobilized self-sufficient monooxygenase has demonstrated to be able to perform Baeyer-Villiger oxidation with efficient cofactor recovery and biocatalyst recycling. The proposed biocatalytic process offers access to valuable molecules with high atom economy and limited waste generation. |
Grants: | European Commission 635734 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-452 |
Note: | Authors also thank COST Action CM 1303-Systems Biocatalysis for financial support and the Servei d'Anàlisi Química of UAB for their assistance on mass spectrometry analyses. Daniela Valencia acknowledges UAB for funding her PhD grant. |
Rights: | Tots els drets reservats. |
Language: | Anglès |
Document: | Article ; recerca ; Versió sotmesa a revisió |
Subject: | Cyclohexanone monooxygenase (CHMO) ; Phosphite dehydrogenase (PTDH) ; Multi-enzymatic reactions ; Biocatalyst immobilization ; Biocatalyst reutilization ; Cofactor recycling |
Published in: | Journal of chemical technology and biotechnology, Vol, 93, issue 4 (April 2018) , p. 985-993, ISSN 1097-4660 |
Preprint 29 p, 692.6 KB |